RSK3 Antibody (1F6) - Azide and BSA Free

Novus Biologicals | Catalog # H00006196-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F6

Format

Azide and BSA Free
View all RSK3 Primary Antibodies »

Product Specifications

Immunogen

RPS6KA2 (AAH02363, 631 a.a. ~ 733 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPWVVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQRRGMKRLTSTRL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23913705). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

RPS6KA2 (1F6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RSK3 Antibody (1F6) - Azide and BSA Free

Western Blot: RSK3 Antibody (1F6) [H00006196-M01]

Western Blot: RSK3 Antibody (1F6) [H00006196-M01]

Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody (M01), clone 1F6.Lane 1: RPS6KA2 transfected lysate(83.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RSK3 Antibody (1F6) [H00006196-M01]

Immunocytochemistry/ Immunofluorescence: RSK3 Antibody (1F6) [H00006196-M01]

Immunocytochemistry/Immunofluorescence: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of monoclonal antibody to RPS6KA2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: RSK3 Antibody (1F6) [H00006196-M01]

Immunohistochemistry-Paraffin: RSK3 Antibody (1F6) [H00006196-M01]

Immunohistochemistry-Paraffin: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.
Western Blot: RSK3 Antibody (1F6) [H00006196-M01]

Western Blot: RSK3 Antibody (1F6) [H00006196-M01]

Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi ( Cat # H00006196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody (M01) clone 1F6 (Cat # H00006196-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: RSK3 Antibody (1F6) [H00006196-M01]

Western Blot: RSK3 Antibody (1F6) [H00006196-M01]

Western Blot: RSK3 Antibody (1F6) [H00006196-M01] - RPS6KA2 monoclonal antibody (M01), clone 1F6 Analysis of RPS6KA2 expression in Hela S3 NE.
Proximity Ligation Assay: RSK3 Antibody (1F6) [H00006196-M01]

Proximity Ligation Assay: RSK3 Antibody (1F6) [H00006196-M01]

Proximity Ligation Assay: RSK3 Antibody (1F6) [H00006196-M01] - Analysis of protein-protein interactions between MAPK3 and RPS6KA2. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for RSK3 Antibody (1F6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RSK3

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Ribosomal Protein S6 Kinase III

Alternate Names

RPS6KA2

Entrez Gene IDs

6196 (Human); 20112 (Mouse); 117269 (Rat)

Gene Symbol

RPS6KA2

OMIM

601685 (Human)

UniProt

AAH02363 (Human)

Additional RSK3 Products

Product Documents for RSK3 Antibody (1F6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RSK3 Antibody (1F6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RSK3 Antibody (1F6) - Azide and BSA Free

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Protocols

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