RUVBL1 Antibody

Novus Biologicals | Catalog # NBP2-20245

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (99%), Chicken (99%), Chimpanzee (100%), Rhesus Macaque (100%), Xenopus (97%), Zebrafish (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RUVBL1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (97%).

Localization

Nucleus matrix; Nucleus; nucleoplasm; Cytoplasm; Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUVBL1 Antibody

Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245]

Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245]

Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Human colon carcinoma, using RUVBL1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat heart. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse colon. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245]

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: RUVBL1 Antibody [NBP2-20245]

Immunoprecipitation: RUVBL1 Antibody [NBP2-20245]

Immunoprecipitation: RUVBL1 Antibody [NBP2-20245] - Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 ug of RUVBL1 antibody. Western blot analysis was performed using RUVBL1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
RUVBL1 Antibody

Western Blot: RUVBL1 Antibody [NBP2-20245] -

RUVBL1/2 do not effect EBOV minigenome activity. (A) Schematic diagram of the EBOV minigenome system. The EBOV minigenome system consists of six plasmids: Four support plasmids encode replication complex components NP, L, VP35, & VP30. The EBOV minigenome plasmid encodes a firefly luciferase reporter gene flanked by the leader & trailer sequences of EBOV, & the plasmid that encodes Renilla luciferase is used for normalization. (B) Minigenome activity upon the knockdown of either RUVBL1, RUVBL2, or in combination. Below are protein levels confirmed by immunoblot. HeLa cells were transfected with 80 nM scrambled siRNA, 30 nM siRNA targeting RUVBL1, or 50 nM siRNA targeting RUVBL2. Twenty-four h after siRNA addition, the minigenome components were transfected. Forty-eight h later, minigenome reporter activity was measured. (C) Overexpression of FLAG-RUVBL1 & HA-RUVBL2 in the EBOV minigenome. HeLa cells were left untransfected, or transfected with vector control (VC), or increasing amounts of FLAG-RUVBL1 (125, 250, & 500 ng) or HA-RUVBL2 (125, 250, & 500 ng). Twenty-four h after exogenous transfection, the minigenome components were transfected. Forty-eight h later, minigenome reporter activity was measured. Data represent mean ± SEM from one representative experiment (n = 3) of at least three experiments (* p < 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RUVBL1 Antibody

Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] -

Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - Endogenous RUVBL1 & RUVBL2 colocalize with HA-NP. HeLa cells were transfected with vector control, HA-NP, or HA-VP35. Twenty-four h later, the cells were fixed & processed for immunofluorescence detection of endogenous RUVBL1 or RUVBL2 in the presence of vector control, HA-NP, or HA-VP35. Representative images of (A) endogenous RUVBL1 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) & (B) endogenous RUVBL2 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) are shown. HA-NP or HA-VP35 (green), RUVBL1/2 (red), & Hoechst 33342 nuclear stain (blue) were visualized by confocal microscopy. Scale bars = 20 µM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RUVBL1 Antibody

Western Blot: RUVBL1 Antibody [NBP2-20245] -

Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (NBP2-20245) diluted at 1:1000.
RUVBL1 Antibody

Western Blot: RUVBL1 Antibody [NBP2-20245] -

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (NBP2-20245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RUVBL1 Antibody

Western Blot: RUVBL1 Antibody [NBP2-20245] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (NBP2-20245) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RUVBL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Knockdown Validated

Reported in scientific literature (PMID: 31018511).

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-20245 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RUVBL1

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation.

Alternate Names

49 kDa TATA box-binding protein-interacting protein, 54 kDa erythrocyte cytosolic protein, EC 3.6.1, EC 3.6.4.12,49 kDa TBP-interacting protein, ECP54, INO80 complex subunit H, INO80HTIP49A, NMP 238, NMP238ECP-54, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB (E coli homolog)-like 1, ruvB-like 1, RuvB-like 1 (E. coli), RVB1, TATA binding protein interacting protein 49 kDa, TIH1, TIP49a, TIP49TAP54-alpha, TIP60-associated protein 54-alpha

Gene Symbol

RUVBL1

UniProt

Q9Y265 (Human)

Additional RUVBL1 Products

Product Documents for RUVBL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RUVBL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RUVBL1 Antibody

Customer Reviews for RUVBL1 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 2 of 2 reviews Showing All
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  • RUVBL1 Antibody
    Name: Agbolahan Sofela
    Application: Western Blot
    Sample Tested: Human Brain Whole Tissue Lysate (Adult Normal), Novus NB820-59177
    Species: Human
    Verified Customer | Posted 09/30/2019
    These have been attached. The protein content of all samples were quantified prior to loading, and standardized at concentrations of 40ug.
    RUVBL1 Antibody NBP2-20245
  • RUVBL1 Antibody
    Name: Tetsuo Kiguchi
    Application: Immunoprecipitation
    Sample Tested: Cancer Cell lysate
    Species: Human
    Verified Customer | Posted 11/30/2017
    RUVBL1 Antibody NBP2-20245

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