S100A10 Antibody - BSA Free

Immunohistochemistry-Paraffin: S100A10 Antibody - BSA Free [NBP1-89370]

Analysis in human lung and skeletal muscle tissues using NBP1-89370 antibody. Corresponding S100A10 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: S100A10 Antibody - BSA Free [NBP1-89370]

Staining of human small intestine shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: S100A10 Antibody - BSA Free [NBP1-89370]

Staining of human skin shows strong membranous positivity in squamous epithelial cells.

Western Blot: S100A10 Antibody - BSA Free [NBP1-89370]

Analysis in human cell lines Caco-2 and HEK293 using Anti-S100A10 antibody. Corresponding S100A10 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Western Blot: S100A10 Antibody - BSA Free [NBP1-89370]

Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-S100A10 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/ Immunofluorescence: S100A10 Antibody - BSA Free [NBP1-89370] -

The AT2-blocker PD123319 induces S100A10 expression in astrocytes with increased proliferation whereas Telmisartan does not affect S100A10 or Ki67 expression. *p < 0.05, **p < 0.01, and ***p < 0.001 compared different experimental groups as marked by horizontal bar; graphs depict mean values +/- standard error of the mean (SEM). A Experimental timeline. Three to five days after subculturing, astrocytes were left unstimulated or treated with 10 uM telmisartan or 10 uM PD123319 for 48 h. They were then exposed to 2 h of oxygen–glucose deprivation (OGD) before they were allowed to recover in regular culture medium for 24 h. Afterward, the cells were used for further experiments. Unstimulated astrocytes, which were exposed to 2 h of OGD, served as controls. B S100A10 marker expression in untreated (unstimulated) or OGD-treated (2 h of OGD) astrocytes with or without the additional preincubation with 10 uM telmisartan or 10 uM PD123319. C Proliferation rate of untreated (unstimulated) and OGD-treated (2 h OGD) astrocytes, with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h as assessed by mRNA-ki67 expression. D ATP concentration of untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h. E Expression of the connexin Cx43 in untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan and 10 uM PD123319 over 48 h. F Representative immunocytochemical stainings of astrocytes with Glia Fibrillary Acidic Protein (GFAP) + (green) and S100A10 + (red) in unstimulated (control), PD123319-preincubated (PD123319), or after 2 h of OGD (OGD + Control). Hoechst stained all cell nuclei blue; scale bars = 50 um Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38926677), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: S100A10 Antibody - BSA Free [NBP1-89370] -

The AT2-blocker PD123319 induces S100A10 expression in astrocytes with increased proliferation whereas Telmisartan does not affect S100A10 or Ki67 expression. *p < 0.05, **p < 0.01, and ***p < 0.001 compared different experimental groups as marked by horizontal bar; graphs depict mean values +/- standard error of the mean (SEM). A Experimental timeline. Three to five days after subculturing, astrocytes were left unstimulated or treated with 10 uM telmisartan or 10 uM PD123319 for 48 h. They were then exposed to 2 h of oxygen–glucose deprivation (OGD) before they were allowed to recover in regular culture medium for 24 h. Afterward, the cells were used for further experiments. Unstimulated astrocytes, which were exposed to 2 h of OGD, served as controls. B S100A10 marker expression in untreated (unstimulated) or OGD-treated (2 h of OGD) astrocytes with or without the additional preincubation with 10 uM telmisartan or 10 uM PD123319. C Proliferation rate of untreated (unstimulated) and OGD-treated (2 h OGD) astrocytes, with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h as assessed by mRNA-ki67 expression. D ATP concentration of untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan or 10 uM PD123319 over 48 h. E Expression of the connexin Cx43 in untreated or OGD-treated astrocytes with or without preincubation with 10 uM telmisartan and 10 uM PD123319 over 48 h. F Representative immunocytochemical stainings of astrocytes with Glia Fibrillary Acidic Protein (GFAP) + (green) and S100A10 + (red) in unstimulated (control), PD123319-preincubated (PD123319), or after 2 h of OGD (OGD + Control). Hoechst stained all cell nuclei blue; scale bars = 50 um Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38926677), licensed under a CC-BY license. Not internally tested by Novus Biologicals.