S100A4 Antibody (1C4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80936

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # 1C4

Format

Azide and BSA Free
View all S100A4 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human S100A4 protein (between amino acids 1-200) [UniProt P26447].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Theoretical MW

11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S100A4 Antibody (1C4) - Azide and BSA Free

Western Blot: S100A4 Antibody (1C4)Azide and BSA Free [NBP2-80936]

Western Blot: S100A4 Antibody (1C4)Azide and BSA Free [NBP2-80936]

Western Blot: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - WB analysis of S100A4 recombinant protein with S100A4 antibody (clone 1C4) at 0.5 ug/ml concentration. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - S100A4 (1C4) antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.
Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - Analysis of tissue section of normal human spleen with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. Image from the standard format of this antibody.
Flow Cytometry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Flow Cytometry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Flow Cytometry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - An intracellular stain was performed on THP-1 cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image from the standard format of this antibody.
Western Blot: S100A4 Antibody (1C4)Azide and BSA Free [NBP2-80936]

Western Blot: S100A4 Antibody (1C4)Azide and BSA Free [NBP2-80936]

Western Blot: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - WB analysis of S100A4 in lysates of A375 and A549 cell lines with S100A4 antibody (clone 1C4). The antibody detected the endogenous protein at its expected molecular weight position 11.7 kDa. Image from the standard format of this antibody.
Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - Aanalysis of tissue section of Squamous Cell Carcinoma of Human Lungs with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. An expected cytoplasmic as well as nuclear staining was observed in the tissue and the staining was found to
Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - Analysis of tissue section of human colon with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The columnar epithelial cells showed weak to negligible immunopositivity whereas the cells present in lamina propria, the loose cellular
Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - Analysis of tissue section of human normal lung with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. Majority of the cells of respiratory alveoli showed the cytoplasmic staining whereas some clusters of cells (lymphocytes, macrophag
Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Immunohistochemistry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - Analysis of tissue section of normal human skeletal muscle with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The different myocytes showed a varying degree of cytoplasmic immuno-positivity whereas the staining was found to be int
Flow Cytometry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Flow Cytometry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Flow Cytometry: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - An intracellular stain was performed on HeLa cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image from the standard format of this antibody.
Knockout Validated: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Western Blot: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936]

Western Blot: S100A4 Antibody (1C4) - Azide and BSA Free [NBP2-80936] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36431) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.

Applications for S100A4 Antibody (1C4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1.0 ug/ml

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A4

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium binding domains. This protein is ubiquitously overexpressed and is localized in the cytoplasm and/or nucleus. S100A4 may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of the S100A4 have been implicated in tumor metastasis. Recombinant mouse S100A4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Gene Symbol

S100A4

Additional S100A4 Products

Product Documents for S100A4 Antibody (1C4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for S100A4 Antibody (1C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for S100A4 Antibody (1C4) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review S100A4 Antibody (1C4) - Azide and BSA Free and earn rewards!

Have you used S100A4 Antibody (1C4) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies