S100A4 Antibody (1F11) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80935

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1F11

Format

Azide and BSA Free
View all S100A4 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human S100A4 protein (between amino acids 1-200) [Uniprot: P26447]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for S100A4 Antibody (1F11) - Azide and BSA Free

Western Blot: S100A4 Antibody (1F11)Azide and BSA Free [NBP2-80935]

Western Blot: S100A4 Antibody (1F11)Azide and BSA Free [NBP2-80935]

Western Blot: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935] - Analysis of S100A4 (1F11) in A375 and A549 cell lysate. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935] - S100A4 (1F11) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.
Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935] - Analysis of formalin-fixed paraffin-embedded tissue section of normal human spleen with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml concentration. Image from the standard format of this antibody.
Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935] - Analysis of FFPE tissue section of human normal lung with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml concentration. Majority of the cells of respiratory alveoli showed the cytoplasmic staining whereas some clusters of cells (lymphocytes, mac
Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Immunohistochemistry: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935] - Analysis of FFPE tissue section of human normal skin with mouse monoclonal S100A4 antibody (clone 1F11) at 5 ug/ml concentration. The epidermal cellular layers as well as the dermal connective tissue showed weak to negligible immunopositivity, however, ve
Knockout Validated: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Western Blot: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935]

Western Blot: S100A4 Antibody (1F11) - Azide and BSA Free [NBP2-80935] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36430) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.

Applications for S100A4 Antibody (1F11) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5 - 1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A4

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium binding domains. This protein is ubiquitously overexpressed and is localized in the cytoplasm and/or nucleus. S100A4 may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of the S100A4 have been implicated in tumor metastasis. Recombinant mouse S100A4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Gene Symbol

S100A4

Additional S100A4 Products

Product Documents for S100A4 Antibody (1F11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for S100A4 Antibody (1F11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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