S100A4 Antibody (1F12-1G7) - Azide and BSA Free

Novus Biologicals | Catalog # H00006275-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F12-1G7

Format

Azide and BSA Free
View all S100A4 Primary Antibodies »

Product Specifications

Immunogen

S100A4 (AAH16300, 1 a.a. ~ 101 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCDEFFEGFPDKQPRKK

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

S100A4 - S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for S100A4 Antibody (1F12-1G7) - Azide and BSA Free

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody (M01), clone 1F12-1G7.Lane 1: S100A4 transfected lysate(11.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Analysis of monoclonal antibody to S100A4 on HeLa cell. Antibody concentration 15 ug/ml.
Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 expression in NIH/3T3 ( Cat # L018V1).
Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 monoclonal antibody (M01), clone 1F12-1G7 Analysis of S100A4 expression in Hela.
Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01]

Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 monoclonal antibody (M01), clone 1F12-1G7. Analysis of S100A4 expression in Raw 264.7.
ELISA: S100A4 Antibody (1F12-1G7) [H00006275-M01]

ELISA: S100A4 Antibody (1F12-1G7) [H00006275-M01]

ELISA: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Detection limit for recombinant GST tagged S100A4 is approximately 0.1ng/ml as a capture antibody.

Applications for S100A4 Antibody (1F12-1G7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF and ELISA. IHC was reported in scientific literature. Use in Immunohistochemistry reported in scientific literature (PMID: 24067916).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: S100A4

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Entrez Gene IDs

6275 (Human)

Gene Symbol

S100A4

OMIM

114210 (Human)

UniProt

AAH16300 (Human)

Additional S100A4 Products

Product Documents for S100A4 Antibody (1F12-1G7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for S100A4 Antibody (1F12-1G7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for S100A4 Antibody (1F12-1G7) - Azide and BSA Free

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Protocols

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FAQs

No product specific FAQs exist for this product.

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