S100A4 Antibody (S01-7H1)
Novus Biologicals | Catalog # NBP3-19607
Recombinant Monoclonal Antibody
![Western Blot: S100A4 Antibody (S01-7H1) [NBP3-19607]](https://resources.rndsystems.com/images/products/S100A4-Antibody-S01-7H1-Western-Blot-NBP3-19607-img0003.jpg "Western Blot: S100A4 Antibody (S01-7H1) [NBP3-19607]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S01-7H1 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide of human S100A4 (Uniprot # P26447)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for S100A4 Antibody (S01-7H1)
Western Blot: S100A4 Antibody (S01-7H1) [NBP3-19607]
Western Blot: S100A4 Antibody (S01-7H1) [NBP3-19607] - Western blot detection of S100A4 in Hela cell lysates using NBP3-19607 (1:500 diluted). Predicted band size: 12kDa. Observed band size: 12kDa.![Immunohistochemistry-Paraffin: S100A4 Antibody (S01-7H1) [NBP3-19607]](https://resources.rndsystems.com/images/products/S100A4-Antibody-S01-7H1-Immunohistochemistry-Paraffin-NBP3-19607-img0002.jpg "Immunohistochemistry-Paraffin: S100A4 Antibody (S01-7H1) [NBP3-19607]")
Immunohistochemistry-Paraffin: S100A4 Antibody (S01-7H1) [NBP3-19607]
Immunohistochemistry-Paraffin: S100A4 Antibody (S01-7H1) [NBP3-19607] - Immunohistochemistry of S100A4 in paraffin-embedded Human lung cancer tissue using NBP3-19607 at dilution 1/50
Immunocytochemistry/Immunofluorescence: S100A4 Antibody (S01-7H1) [NBP3-19607] -
Immunocytochemistry/Immunofluorescence: S100A4 Antibody (S01-7H1) [NBP3-19607] - Immunocytochemistry of S100A4 (green) in hela using NBP3-19607 at dilution 1/5, and DAPI (blue)Applications for S100A4 Antibody (S01-7H1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1/20-1/50
Immunohistochemistry
1/50-1/200
Western Blot
1/1000-1/5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: S100A4
Long Name
S100 Calcium Binding Protein A4
Alternate Names
18A2, 42A, CAPL, MTS1, P9KA, PEL98
Gene Symbol
S100A4
Additional S100A4 Products
Product Documents for S100A4 Antibody (S01-7H1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for S100A4 Antibody (S01-7H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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