S100A4 Antibody

Novus Biologicals | Catalog # NB100-55404

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all S100A4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CNEFFEGFPDKQP corresponding to C-Terminus according to NP_062427.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31024074).

Localization

Nuclear

Specificity

Both variants (NP_002952.1; NP_062427.1) represent identical protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S100A4 Antibody

Western Blot: S100A4 Antibody [NB100-55404]

Western Blot: S100A4 Antibody [NB100-55404]

Western Blot: S100A4 Antibody [NB100-55404] - (0.05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: S100A4 Antibody [NB100-55404]

Immunohistochemistry-Paraffin: S100A4 Antibody [NB100-55404]

Immunohistochemistry-Paraffin: S100A4 Antibody [NB100-55404] - (2ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for S100A4 Antibody

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.05-0.2 ug/ml
Application Notes
WB: Approx. 13 kDa band observed in human placenta lysates (calculated MW of 11.7 kDa band according to NP_002952.1and NP_062427.1). IHC-P: Human small intestine shows strong nuclear staining in some cells and weaker cytoplasm staining in some other cells. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 31024074).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S100A4

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Entrez Gene IDs

6275 (Human); 20198 (Mouse); 24615 (Rat)

Gene Symbol

S100A4

UniProt

P26447

Additional S100A4 Products

Product Documents for S100A4 Antibody

Certificate of Analysis

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Product Specific Notices for S100A4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for S100A4 Antibody

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Protocols

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