S100A7/Psoriasin Antibody (47C1068) - BSA Free

Immunohistochemistry: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559]

S100A7-Psoriasin-Antibody-47C1068-Immunohistochemistry-NB100-56559-img0019.jpg

Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559]

Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.. Using the PE format of this antibody.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559]

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Staining of human skin. FFPE tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/mL.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559]

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone 47C1068). Diffused to granular cytoplasmic immunostaining was observed all over the tissue section with highest expression levels in the areas with cancer cells especially in the developing squamous keratin pearls.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559]

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis of FFPE tissue section of human lymph node cancer using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone 47C1068). Very strong cytoplasmic immunopositivity of Psoriasin/S100A7 protein was observed in the cancer cells.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559]

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Human breast tissue stained with psoriasin antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Antigen retrieval with citrate buffer, pH 6.

Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559]

Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Simple Western: S100A7/Psoriasin Antibody (47C1068)BSA Free [NB100-56559]

Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Image shows a specific band for S100A7/Psoriasin in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: S100A7/Psoriasin Antibody (47C1068)BSA Free [NB100-56559]

Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Image shows a specific band for Psoriasin/S100A7 in 0.05 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] -

S100A7 activated p53 related anti-proliferative pathways. (A) Log2 fold change of mRNA expression of p53 pathway factors after S100A7 overexpression. (B) WB of p53 and S100A7 in MCF7-p53-CKD transfected with S100A7 or vector control. p53 shRNA transcription and p53 knockdown in MCF7-p53-CKD was induced by Doxycyclin (Dox). (C) S100A7 activated p53, ATR, chk-1, chk-2 in MCF7 cells.

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] -

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 activated p53 related anti-proliferative pathways. (A) Log2 fold change of mRNA expression of p53 pathway factors after S100A7 overexpression. (B) WB of p53 & S100A7 in MCF7-p53-CKD transfected with S100A7 or vector control. p53 shRNA transcription & p53 knockdown in MCF7-p53-CKD was induced by Doxycyclin (Dox). (C) S100A7 activated p53, ATR, chk-1, chk-2 in MCF7 cells. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25622979), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] -

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 induces EMT in cervical cancer cellsWestern Blot showed the protein level of epithelial marker E-cadherin & mesenchymal markers (N-Cadherin, Vimentin, Fibronectin) & EMT transcription factors Snail & Slug after overexpression of S100A7 in C33A cells A. & SiHa cells B. beta -actin is used as a loading control. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.15329), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] -

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 interacts with RAGE, stimulates ERK pathway & RAGE mediates S100A7-induced migration & invasion in C33A & SiHa cellsA. HEK293 cells were transiently co-expressed with pcDNA3-Flag & pcDNA3-Flag-RAGE with pcDNA3-myc-S100A7. Cell extracts were immunoprecipitated separately with specific antibody against Flag, & the associated Flag-RAGE & myc-S100A7 were examined by Western blotting using tagged antibodies, respectively. B. S100A7-expressing cells were found to have higher levels of phospho-ERK (without any change in total ERK levels). In comparison, the levels of phospho-Akt & phospho-NF-kappa B had no obvious change. C. qRT-PCR verification of RAGE knockdown by two independent siRNAs in C33A & SiHa cells. D&E. RAGE knockdown suppressed migration & invasion in S100A7-overexpressed C33A & SiHa cells & their corresponding control cells. The stained cells were manually counted from 4 randomly selected fields & normalized with cell proliferation [2-sided t test; * P < 0.05; **P < 0.01]. Representative image & quantitative results of cell migration & invasion were shown (D. C33A cells; E. SiHa cells; ×10, bars: 100μm). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.15329), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] -

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 is secreted & acts as a chemotactic factor of cell migration & invasionA. Cells were cultured in serum-free medium for 2 days, then the medium was collected, fractioned, followed by Western Blot analysis. B&C. Transwell migration & Matrigel invasion assays were performed in C33A & SiHa cells. Cell suspension was placed into the upper chamber in 0.1 ml of DMEM serum-free medium, & conditioned medium from S100A7-overexpressed C33A & SiHa cells & their corresponding control cells was placed in the lower chamber as a chemoattractant. The stained cells were manually counted from 4 randomly selected fields & normalized with cell proliferation [2-sided t test; *P < 0.05; ***P < 0.001]. Representative image & quantitative results of cell migration & invasion were shown (B. C33A cells; C. SiHa cells; ×10, bars:100μm). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.15329), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] -

Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 promotes cervical cancer cell migration & invasionA&B. Establishment of stable cell lines of ectopic expression of S100A7. C33A & SiHa cells were infected with pLVX-Con & pLVX-S100A7 lentivirus, stable cells were established by Geneticin (G418) selection for about 2 weeks. Cells were harvested, S100A7 expression was detected by qRT-PCR (A: [mean (n=2) ± SD; 2-sided t test; ** P<0.01, normalized to beta -actin]) & Western Blot. C&D. Transwell migration & Matrigel invasion assays were performed in S100A7-overexpressed cells & their corresponding control cells. The stained cells were manually counted from 4 randomly selected fields & normalized with cell proliferation [2-sided t test; **P < 0.01]. Representative image & quantitative results of cell migration & invasion were shown (C. C33A cells; D. SiHa cells; ×10, bars:100μm). E. The effect of S100A7 on cell migration was examined in wound-healing assay. Confluent cells were scratched & photographed at time 0h & 48h (Left panel. C33A cells; Right panel. SiHa cells. ×10, bars:100μm). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.15329), licensed under a CC-BY license. Not internally tested by Novus Biologicals.