SART3 Antibody

Novus Biologicals | Catalog # NB100-74648

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SART3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human squamous cell carcinoma antigen recognized by T-cells 3 (Tat-interacting protein of 110 kDa) (NP_055521.1).

Localization

Cytoplasmic and Nuclear. Localized in speckles.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SART3 Antibody

Western Blot: SART3 Antibody [NB100-74648]

Western Blot: SART3 Antibody [NB100-74648]

Western Blot: SART3 Antibody [NB100-74648] - Detection of Human SART3/TIP110 on HeLa whole cell lysate using NB100-74648. SART3 was also immunoprecipitated by rabbit anti-SART3 antibody NB100-74649.
Immunocytochemistry/ Immunofluorescence: SART3 Antibody [NB100-74648]

Immunocytochemistry/ Immunofluorescence: SART3 Antibody [NB100-74648]

Immunocytochemistry/Immunofluorescence: SART3 Antibody [NB100-74648] - FFPE section of human pancreatic islet cell tumor. Affinity purified rabbit anti-SART3 used at a dilution of 1:100. Detection: Red fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.
Immunohistochemistry: SART3 Antibody [NB100-74648]

Immunohistochemistry: SART3 Antibody [NB100-74648]

Immunohistochemistry: SART3 Antibody [NB100-74648] - Sample: FFPE sections of human ovarian carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- SART3/TIP110 used at a dilution of 1:200 (1ug/ml) and 1:400 (0.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG
Immunohistochemistry-Paraffin: SART3 Antibody [NB100-74648]

Immunohistochemistry-Paraffin: SART3 Antibody [NB100-74648]

Immunohistochemistry-Paraffin: SART3 Antibody [NB100-74648] - FFPE section of human pancreatic islet cell tumor. Affinity purified rabbit anti-SART3 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: SART3 Antibody [NB100-74648]

Immunohistochemistry-Paraffin: SART3 Antibody [NB100-74648]

Immunohistochemistry-Paraffin: SART3 Antibody [NB100-74648] - FFPE section of human pancreatic islet cell tumor. Affinity purified rabbit anti-SART3 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

Applications for SART3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250- 1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SART3

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.

Alternate Names

DSAP1, hSART-3, KIAA0156squamous cell carcinoma antigen recognised by T cells 3, P100, p110, p110(nrb), RP11-13G14, SART-3, squamous cell carcinoma antigen recognized by T cells 3, squamous cell carcinoma antigen recognized by T-cells 3, Tat-interacting protein of 110 kDa, Tip110, TIP110MGC138188

Entrez Gene IDs

9733 (Human)

Gene Symbol

SART3

UniProt

Q15020 (Human)

Additional SART3 Products

Product Documents for SART3 Antibody

Certificate of Analysis

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Product Specific Notices for SART3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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