SART3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31487

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SART3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SART3. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus speckle

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SART3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SART3 Antibody [NBP1-31487]

Immunocytochemistry/ Immunofluorescence: SART3 Antibody [NBP1-31487]

Immunocytochemistry/Immunofluorescence: SART3 Antibody [NBP1-31487] - Analysis of HeLa, using NBP1-31487 at 1:200 dilution.
Immunohistochemistry-Paraffin: SART3 Antibody [NBP1-31487]

Immunohistochemistry-Paraffin: SART3 Antibody [NBP1-31487]

Immunohistochemistry-Paraffin: SART3 Antibody [NBP1-31487] - NCI-N87 xenograft, using SART3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: SART3 Antibody [NBP1-31487]

Immunoprecipitation: SART3 Antibody [NBP1-31487]

Immunoprecipitation: SART3 Antibody [NBP1-31487] - ART3 antibody [C3], C-term immunoprecipitates SART3 protein in IP experiments.IP samples: HepG2 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of SART3 protein by 4 ug SART3 antibody [C3], C-term. 5 % SDS-PAGE. The immunoprecipitated SART3 protein was detected by SART3 antibody [C3], C-term diluted at 1:2000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
SART3 Antibody

Western Blot: SART3 Antibody [NBP1-31487] -

Western Blot: SART3 Antibody [NBP1-31487] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (NBP1-31487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SART3 Antibody - BSA Free

Western Blot: SART3 Antibody [NBP1-31487] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (NBP1-31487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for SART3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SART3

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.

Alternate Names

DSAP1, hSART-3, KIAA0156squamous cell carcinoma antigen recognised by T cells 3, P100, p110, p110(nrb), RP11-13G14, SART-3, squamous cell carcinoma antigen recognized by T cells 3, squamous cell carcinoma antigen recognized by T-cells 3, Tat-interacting protein of 110 kDa, Tip110, TIP110MGC138188

Gene Symbol

SART3

Additional SART3 Products

Product Documents for SART3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SART3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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