Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-52337

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # K74/71

Format

Azide and BSA Free
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Product Specifications

Immunogen

Fusion protein amino acids 1929-2009 (cytoplasmic C-terminus) of rat Scn1a (Uniprot#: P04774) produced recombinantly in E. Coli

Specificity

No cross-reactivity with Nav1.2, Nav1.3 and Nav1.8

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K74/71 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K74/71 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Scientific Data Images for Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free

Immunohistochemistry-Paraffin: Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free [NBP3-52337]

Immunohistochemistry-Paraffin: Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free [NBP3-52337]

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing cells stained by NBP3-52336 (the BSA Free version of this antibody) using goat anti-chicken IgY secondary antibody (visualized in green). DAPI nuclear stain (blue) shows cell nuclei.
Western Blot: Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free [NBP3-52337]

Western Blot: Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free [NBP3-52337]

Western blotting of rat brain membrane with NBP3-52336 (the BSA Free version of this antibody). The chicken mAb was detected with anti-chicken HRP. This antibody recognizes endogenous Scn1a in the rat brain membrane fraction at ~220 kDa.
Immunohistochemistry-Paraffin: Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free [NBP3-52337]

Immunohistochemistry-Paraffin: Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free [NBP3-52337]

Representative section of formalin fixed, paraffin-embedded rat brain hippocampal CA2/CA3 region showing cells stained by NBP3-52336 (the BSA Free version of this antibody) using goat anti-chicken IgY secondary antibody (visualized in green). DAPI nuclear stain (blue) shows cell nuclei.

Applications for Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Scn1a

Voltage-dependent sodium channels are heteromeric complexes that regulate sodium exchange between intracellular and extracellular spaces and are essential for the generation and propagation of action potentials in muscle cells and neurons. Each sodium channel is composed of a large pore-forming, glycosylated alpha subunit and two smaller beta subunits. This gene encodes a sodium channel alpha subunit, which has four homologous domains, each of which contains six transmembrane regions. Allelic variants of this gene are associated with generalized epilepsy with febrile seizures and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript. [provided by RefSeq, Oct 2015]

Alternate Names

brain I alpha subunit, DEE6, DEE6A, DEE6B, DRVT, EIEE6, FEB3, FEB3A, FHM3, GEFSP2, HBSCI, NAC1, Nav1.1, SCN1, SMEI, sodium channel, voltage-gated, type I, alpha polypeptide, sodium channel, voltage-gated, type I, alpha subunit

Gene Symbol

SCN1A

Additional Scn1a Products

Product Documents for Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Scn1a Antibody (K74/71) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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