SCP1 Antibody - BSA Free

Novus Biologicals | Catalog # NB300-229

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Chicken, Human (Negative), Mammal, Monkey, Parasite

Cited:

Human, Mouse, Rat, Mammal, Parasite, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SCP1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the C-terminus of the mouse SCP1 protein sequence. [UniProt# Q62209]

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 25981592). Parasite reactivity reported in scientific literature (PMID: 27084479). Chicken reactivity reported in scientific literature (PMID: 28174243). Use in Monkey reported in scientific literature (PMID: 31907447). This antibody has not been shown to have human reactivity.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCP1 Antibody - BSA Free

Simple Western: SCP1 AntibodyBSA Free [NB300-229]

Simple Western: SCP1 AntibodyBSA Free [NB300-229]

Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: SCP1 AntibodyBSA Free [NB300-229]

Western Blot: SCP1 AntibodyBSA Free [NB300-229]

Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), which are axial element and central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) and abnormal SYCP3 structures (arrows). Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS One (2016)
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

SCP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB300-229-img0017.jpg
Immunohistochemistry-Paraffin: SCP1 Antibody - BSA Free [NB300-229]

Immunohistochemistry-Paraffin: SCP1 Antibody - BSA Free [NB300-229]

Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - SCP1 labeled in mouse pachytene preparation (red), using NB300-229. CDK2 staining, near teleomeres, is also present (green).
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Spermatocytes cells fixed in PFA. Detected with anti-mouse 594. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

SCP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB300-229-img0016.jpg
SCP1 Antibody - BSA Free

Chromatin Immunoprecipitation: SCP1 Antibody - BSA Free [NB300-229] -

Chromatin Immunoprecipitation: SCP1 Antibody - BSA Free [NB300-229] - Meiotic spreads.Meiotic chromosome spreads from wild type & Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) & SYCP1 (green), which are axial element & central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) & abnormal SYCP3 structures (arrows). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0159800), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SCP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Meiotic progression & chromosome paring in XX, XO & XY oocytes.(A) Meiotic progression in XX, XO & XY oocytes. The graphs show the percentages of the meiotic stage at the day of culture indicated. L, leptotene; Z, zygotene; P, pachytene; D, diplotene. (B) Pairing of homologous chromosomes in XX, XO & XY oocytes. Images show the immunofluorescence analysis of SYCP3 (white) & SYCP1 (red), & FISH analysis of the X chromosome (green) & Y chromosome (purple). The dashed squares in the merged images are shown at high magnification (right). The numbers of samples showing the phenotype are shown with the total number tested (left). Arrowheads indicate asynapsed bivalents at the end of the chromosomes. Scale bars, 10 μm. (C) Pairing rates of autosomes & sex chromosomes. Each value was calculated from three independent experiments (see also Materials & Methods). P values were calculated by Tukey’s HSD test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214314), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SCP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Meiotic prophase I progression & apoptosis in spermatocytes in ex vivo culture at different temperatures.a Representative images of chromosomal spreads at different stages of meiotic prophase I, prepared from in vivo-developed testes of 5-week-old Acr-GFP mice & from testis explants cultured at 34, 37, or 38 °C for 5 weeks as indicated. Samples were stained for SCP3, SCP1, & DNA (Hoechst 33342). Scale bars, 10 μm. b Proportions of spermatocytes in the leptotene (lep), zygotene (zygo), pachytene (pachy), & diplotene (diplo) stages, found in explants cultured for 5 weeks at the indicated temperatures classified visually on the chromosome spreads after immunofluorescence-staining for SCP1 & SCP3 as (a), according to the criteria described in the text. Values obtained from chromosome spreads prepared from pooled testicular cells of two in vivo-developed testes from different individuals & those from 6 to 7 ex vivo-grown explants are summarized. The total number of spermatocyte nuclei counted is indicated at the upper right of each panel. Percentages of spermatocytes nuclei in which all the autosomes have completed synapsis (i.e., the sum of pachytene & diplotene spermatocytes) were 63, 52, 16, & 0.5% for in vivo & ex vivo samples at 34, 37, & 38 °C, respectively. c Detection of cleaved Caspase-3 (red) & SCP3 (green) in testis explants following the temperature shift from 34 to 38 °C. Double-stained images overlaid with DNA staining (gray) & the signals for cleaved Caspase-3 alone are shown in the upper & lower panels, respectively. Enlarged images at positions indicated by rectangles are also shown below. Yellow arrowheads, Caspase-3+/SCP3+ double-positive cells (the dying spermatocytes). Scale bars, 40 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35618762), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SCP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - No major meiotic DSB repair or chromosome synapsis defects are observed in Cxxc1 CKO testis.(A) Immunostaining of SYCP3 & gamma H2AX on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; magenta, gamma H2AX. Scale bar, 10 μm. (B) Spermatocyte stage proportion in adult Cxxc1 het (n = 1,062 from two individuals) & CKO (n = 1,105 from two individuals) spermatocytes based on SYCP3/SYCP1/ gamma H2AX staining. p = 0.7 by Chi-square test. (C) Immunostaining of SYCP3 & SYCP1 on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; orange, SYCP1. Scale bar, 10 μm. (D) Crossover number measured by MLH1 staining on chromosome spreads of adult Cxxc1 het & CKO spermatocytes. Left, magenta, SYCP3; green, MLH1. Scale bar, 10 μm. Right, number of MLH1 foci per late pachynema in Cxxc1 het (n = 32 from two individuals) & CKO (n = 33 from two individuals). Bars represent mean ± SD. p = 0.4 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SCP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - UHRF1 deletion disrupted the meiotic progression & synaptonemal complex assembly.a Relative amounts of four spermatocyte populations (leptotene stage, zygotene stage, pachytene stage, & diplotene stage) during the prophase I in testes based on analyzing >600 spermatocytes in each stage. b, c The immunostaining of SYCP3 in the testicular sections (b) & surface-spread chromatin preparations of Uhrf1 deletion & control mice (c); d the percentage of spermatocytes with abnormal SYCP3 location. e Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) & SYCP1 (red). f The percentage of spermatocytes with abnormal SYCP1 location. Lep leptotene, Zyg zygotene, Pac pachytene, Dip diplotene. Data are presented as mean ± SEM of three mice. ***p ≤ 0.001. Scale bar, 25 μm in b, 5 μm in c, e. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32081844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SCP1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10 - 1:500. Use reported in scientific literature (PMID 27486799)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:100 - 1:750

Immunohistochemistry

1:750

Immunohistochemistry-Frozen

1:750

Immunohistochemistry-Paraffin

1:750

Simple Western

1:100
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Testis and Mouse Testis lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 3 reviews rated 5 using NB300-229 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCP1

Synaptonemal complex protein 1 is a major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. SCP1 is equired for normal assembly of the central element of the synaptonemal complexes, as well as for normal centromere pairing during meiosis.

Alternate Names

Cancer/testis antigen 8, CT8MGC104417, HOM-TES-14, SCP1SCP-1, synaptonemal complex protein 1

Gene Symbol

SYCP1

Additional SCP1 Products

Product Documents for SCP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SCP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SCP1 Antibody - BSA Free

Customer Reviews for SCP1 Antibody - BSA Free (3)

5 out of 5
3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • SCP1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: spermatocyte
    Species: Mouse
    Verified Customer | Posted 02/17/2021
    Red colour is scp1 colocalize with scp3(green)
    SCP1 Antibody - BSA Free NB300-229
  • SCP1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mouse spermatozoa
    Species: Mouse
    Verified Customer | Posted 09/12/2019
    green colour is scp1 staining. It's good for IF.
    SCP1 Antibody - BSA Free NB300-229
  • SCP1 Antibody
    Name: Daniela Moralli
    Application: Immunocytochemistry
    Sample Tested: Spermatocytes
    Species: Mouse
    Verified Customer | Posted 04/11/2017
    Spermatocytes cells fixed in PFA. Detected with anti-mouse 594
    SCP1 Antibody - BSA Free NB300-229

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Protocols

View specific protocols for SCP1 Antibody - BSA Free (NB300-229):


Immunofluorescence Procedure
1. Freshly prepared slides are soaked in 1X ADB for 75 minutes.
2. Primary antibodies are added concurrently (SCP1 and CDK2).
3. The primary antibodies are incubated overnight in a hudid chamber (37 degrees Celcius).
4. The slides are washed for 40 minutes in 1X ADB.
5. The slides are detected with the appropriate secondary antibodies (RDAR for SCP1 and FDAM for CDK2).
6. The slides are incubated for 4 hours in a humid chamber (37 degrees Celcius).
7. The slides are washed for 20 minutes in 1X ADB, followed by 3 washes, 10 minutes each, in 1X PBS.
8. The slides are counterstained with DAPI.
9. Images are captured after allowing the slides to remain in the dark overnight at RT.

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