SEC23IP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82456

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SEC23IP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HLELKESLSRMGSDLKQGFISSLKSAWQTLNEFARAHTSSTQLQEELEKVANQIKEEEEKQVVEAEKVVESPDFSKDEDYLGKVGML

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC23IP Antibody - BSA Free

Western Blot: SEC23IP Antibody [NBP1-82456]

Western Blot: SEC23IP Antibody [NBP1-82456]

Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: SEC23IP Antibody [NBP1-82456]

Western Blot: SEC23IP Antibody [NBP1-82456]

Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SEC23IP Antibody [NBP1-82456]

Immunocytochemistry/ Immunofluorescence: SEC23IP Antibody [NBP1-82456]

Immunocytochemistry/Immunofluorescence: SEC23IP Antibody [NBP1-82456] - Staining of human cell line A-431 shows positivity in vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456]

Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456]

Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human tonsil shows moderate granular cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456]

Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456]

Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456]

Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456]

Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456]

Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456]

Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.

Applications for SEC23IP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SEC23IP

COPII-coated vesicles are involved in protein transport from the endoplasmic reticulum to the Golgi apparatus. The protein encoded by this gene was identified by its interaction with a mouse protein similar to yeast Sec23p, an essential component of the COPII. This protein shares significant similarity with phospholipid-modifying proteins, especially phosphatidic acid preferring-phospholipase A1. Overexpression of this protein has been shown to cause disorganization of the endoplasmic reticulum-Golgi intermediate compartment and Golgi apparatus, which suggests its role in the early secretory pathway.

Alternate Names

p125, P125A, SEC23 interacting protein, SEC23-interacting protein

Gene Symbol

SEC23IP

Additional SEC23IP Products

Product Documents for SEC23IP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SEC23IP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SEC23IP Antibody - BSA Free

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Protocols

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FAQs

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