SEL1L Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93746

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SEL1L Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-190 of human SEL1L (NP_005056.3). SSDEEGSQDESLDSKTTLTSDESVKDHTTAGRVVAGQIFLDSEESELESSIQEEEDSLKSQEGESVTEDISFLESPNPENKDYEEPKKVRKPALTAIEGTAHGEPCHFPFLFLDKEYDECTSDGREDGRLWCATTYDYKADEKWGFCETEEEAAKRRQMQEAEMMYQTG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SEL1L Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SEL1L Antibody - Azide and BSA Free [NBP2-93746]

Immunocytochemistry/ Immunofluorescence: SEL1L Antibody - Azide and BSA Free [NBP2-93746]

Immunocytochemistry/Immunofluorescence: SEL1L Antibody [NBP2-93746] - Analysis of L929 cells using SEL1L at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SEL1L Antibody - Azide and BSA Free [NBP2-93746]

Immunocytochemistry/ Immunofluorescence: SEL1L Antibody - Azide and BSA Free [NBP2-93746]

Immunocytochemistry/Immunofluorescence: SEL1L Antibody [NBP2-93746] - Analysis of C6 cells using SEL1L at dilution of 1:100. Blue: DAPI for nuclear staining.
SEL1L Antibody - BSA Free

SEL1L Antibody [NBP2-93746] -

Analysis of paraffin-embedded rat testis tissue using SEL1L Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
SEL1L Antibody - BSA Free

SEL1L Antibody [NBP2-93746] -

Analysis of various lysates using SEL1L Rabbit pAb at 1:2000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 45 s.
SEL1L Antibody - BSA Free

SEL1L Antibody [NBP2-93746] -

Analysis of Mouse pancreas tissue using SEL1L Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

Applications for SEL1L Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:400 - 1:1600

Immunohistochemistry-Paraffin

1:400 - 1:1600

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SEL1L

SEL1L may play a role in Notch signaling. May be involved in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins

Alternate Names

IBD2, PRO1063, protein sel-1 homolog 1, sel-1 (suppressor of lin-12, C.elegans)-like, sel-1 suppressor of lin-12-like (C. elegans), sel-1 suppressor of lin-12-like 1, sel-1L, SEL1L1, SEL1-LIKE, Suppressor of lin 12 (sel-1), C. elegans, homolog of, Suppressor of lin-12-like protein 1, TSA305

Gene Symbol

SEL1L

Additional SEL1L Products

Product Documents for SEL1L Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SEL1L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Citations for SEL1L Antibody - BSA Free

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Protocols

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