SELS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89558

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SELS Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAKKPQEEDSPGPSTSSVLKRKSDRKPLRGGGYNPLSGEGGGACSWRPGR

Reactivity Notes

Mouse, Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SELS Antibody - BSA Free

SELS Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: SELS Antibody - BSA Free [NBP1-89558]

Immunocytochemistry/ Immunofluorescence: SELS Antibody - BSA Free [NBP1-89558]

Staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558]

Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558]

Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558] - Staining of human tonsil shows strong cytoplasmic positivity in a small subset of germinal center cells.
Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558]

Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558]

Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558]

Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558]

Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558]

Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558]

Immunohistochemistry-Paraffin: SELS Antibody [NBP1-89558] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
SELS Antibody - BSA Free Western Blot: SELS Antibody - BSA Free [NBP1-89558]

Western Blot: SELS Antibody - BSA Free [NBP1-89558]

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SELENOS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
SELS Antibody - BSA Free Western Blot: SELS Antibody - BSA Free [NBP1-89558]

Western Blot: SELS Antibody - BSA Free [NBP1-89558]

Analysis in human cell line HEK 293.

Applications for SELS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SELS

SELS encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Studies suggest that this protein may regulate cytokine production, and thus play a key role in the control of the inflammatory response. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

ADO15, MGC104346, SBBI8, selenoprotein S, SelS, SEPS1, VCP-interacting membrane protein, VIMPMGC2553

Gene Symbol

VIMP

Additional SELS Products

Product Documents for SELS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SELS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SELS Antibody - BSA Free

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Protocols

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