Septin-9 Antibody - BSA Free

4.5 out of 5

2 Customer Ratings

5 Stars

50%

4 Stars

50%

3 Stars

0%

2 Stars

0%

1 Stars

0%

Have you used Septin-9 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Customer Images

• 
• 
• (2)

• Septin-9 Antibody

  Name: Stephen Terry

  Application: Immunocytochemistry

  Sample Tested: hela cell

  Species: Human

  Verified Customer | Posted 10/25/2018

  IF images of HeLa cells fixed with PFA with anti septin 9 antibody NBP1-28711 1:1000. Left Control localisation to sub nuclear filaments. Right cells treated with cytochalasin D to depolymerize F-actin. Note localization to ring like structures.

  Cells fixed with 4% PFA for 20 minutes room temperature. Fixed cells incubated with with 0.5% triton x100 in 1xPBS for 5 minutes room. Washed twice in blocking buffer (1% BSA 1xPBS, 20mM glycine ). Washed once with blocking buffer left for 1hour room temperature. incubated with Anti septin 9 antibody in blocking buffer 1:1000 for 20 hours at 4 degrees. Washed 3 x 5 minutes in blocking buffer Incubated with Anti rabbit-Alexa 594 conjugated antibody (Jackson immunoscience) for 2 hours room temperature. Washed 3 x 5 minutes in blocking buffer Washed 1 x 5 minutes in PBS Imaged with 60X oil objective on Nikon Eclipse Ti Inverted Fast confocal - Equipped with a Yokogawa CSU-X1 spinning disk unit and okogawa CSU-1 disk head and Andor Neo sCMOS camera.

  

• Septin-9 Antibody

  Name: Stephen Terry

  Application: Western Blot

  Sample Tested: Hela whole cell lysate

  Species: Human

  Verified Customer | Posted 10/22/2018

  lane 1 marker lane 2 10mg of HeLa whole cell lysate probed with NBP1-28711 antibody 1:1000 in PBST

  10mg of HeLa whole cell lysate was loaded run on a 4-12% bi-Tris acrylamide gell run in 1X mops buffer. Blot blocked with 5%milk in PBS with 0.1% tween 20 probed overnight at 4 degrees C with NBP1-28711 1:1000 washed 3X PBST incubated with anti rabbit HRP secondary washed 3X PBST, developed with ECL prime kit GE healthcare.

  

There are no reviews that match your criteria.