Serine Palmitoyltransferase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76573

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Serine Palmitoyltransferase 2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Serine Palmitoyltransferase 2. The immunogen is located within the last 50 amino acids of Serine Palmitoyltransferase 2.

Specificity

Serine Palmitoyltransferase 2 antibody is predicted to not cross-react with SPT1. Human Serine Palmitoyltransferase 2 has one isoform (562aa, 63kD). Mouse Serine Palmitoyltransferase 2 has one isoform (560aa, 63kD) and Rat Serine Palmitoyltransferase 2 also has one isoform (560aa, 63kD). NBP1-76573 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serine Palmitoyltransferase 2 Antibody - BSA Free

Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Mouse 3T3/NIH Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - 3T3 cell lysate with Serine Palmitoyltransferase 2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (1 ug/ml), Serine Palmitoyltransferase 2 from Company B (1 ug/ml), and beta-actin (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 AntibodyBSA Free [NBP1-76573]

Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Human Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Serine Palmitoyltransferase 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Serine Palmitoyltransferase 2 Antibody - BSA Free [NBP1-76573] -

Immunocytochemistry/ Immunofluorescence: Serine Palmitoyltransferase 2 Antibody - BSA Free [NBP1-76573] - Validation of Serine Palmitoyltransferase 2 in Mouse 3T3 Cells. Immunocytochemical analysis of 3T3 cells using anti-Serine Palmitoyltransferase 2 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Serine Palmitoyltransferase 2 Antibody - BSA Free

Western Blot: Serine Palmitoyltransferase 2 Antibody - BSA Free [NBP1-76573] -

Western Blot: Serine Palmitoyltransferase 2 Antibody - BSA Free [NBP1-76573] - Validation in Mouse 3T3 Cell Lysate in (A) the absence and (B) the presence of blocking peptide. Loading: 15 ug of lysates per lane.Antibodies: Serine Palmitoyltransferase 2, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Serine Palmitoyltransferase 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Serine Palmitoyltransferase 2 Antibody - BSA Free [NBP1-76573] -

Immunocytochemistry/ Immunofluorescence: Serine Palmitoyltransferase 2 Antibody - BSA Free [NBP1-76573] - Validation of Serine Palmitoyltransferase 2 in Mouse 3T3 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 Cells labeling Serine Palmitoyltransferase 2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for Serine Palmitoyltransferase 2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

0.5-1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serine Palmitoyltransferase 2

Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.

Long Name

Serine palmitoyltransferase 2

Alternate Names

EC 2.3.1.50, KIAA0526, LCB 2, LCB2, LCB2a, SPT 2, SPTLC2

Gene Symbol

SPTLC2

Additional Serine Palmitoyltransferase 2 Products

Product Documents for Serine Palmitoyltransferase 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Serine Palmitoyltransferase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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