Serpin B5/Maspin Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-87780
![Knockdown Validated: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Knockdown-Validated-NBP1-87780-img0024.jpg "Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]")
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Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Rat
Predicted:
Mouse (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Serpin B5/Maspin Antibody - BSA Free
Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]
Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MASPIN/Serpin B5 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human MASPIN/Serpin B5 Polyclonal Antibody (Catalog # NBP1-87780) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MASPIN/Serpin B5 at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0021.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0012.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human cerebral cortex, liver, skin and testis using Anti-SERPINB5 antibody NBP1-87780 (A) shows similar protein distribution across tissues to independent antibody NBP1-87779 (B).![Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87780-img0022.jpg "Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]")
![Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87780-img0010.jpg "Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]")
![Immunocytochemistry/ Immunofluorescence: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87780-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunocytochemistry/ Immunofluorescence: Serpin B5/Maspin Antibody [NBP1-87780]
Immunocytochemistry/Immunofluorescence: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0020.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human cerebral cortex.![Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Western-Blot-NBP1-87780-img0011.jpg "Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]")
Western Blot: Serpin B5/Maspin Antibody [NBP1-87780]
Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0014.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human testis.![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0015.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human liver.![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0016.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human skeletal muscle shows no positivity in myocytes as expected.![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0017.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0018.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human skin shows strong positivity in squamous epithelial cells.![Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]](https://resources.rndsystems.com/images/products/Serpin-B5-Maspin-Antibody-Immunohistochemistry-Paraffin-NBP1-87780-img0019.jpg "Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]")
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780]
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human upper gastrointestinal shows weak to moderate cytoplasmic/membranous positivity in glandular cells.Applications for Serpin B5/Maspin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Serpin B5/Maspin
Alternate Names
Maspin, PI5
Gene Symbol
SERPINB5
Additional Serpin B5/Maspin Products
Product Documents for Serpin B5/Maspin Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Serpin B5/Maspin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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