Serpin E1/PAI-1 Antibody (1D5) - BSA Free

Western Blot: Serpin E1/PAI-1 Antibody (1D5)BSA Free [NBP2-37532]

Western Blot: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Western blot analysis using SERPINE1 mAb against human SERPINE1 (AA: 194-316) recombinant protein. (Expected MW is 45kDa kDa)

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody (1D5) - BSA Free [NBP2-37532]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Analysis of lung cancer tissues using SERPINE1 mouse mAb with DAB staining.

ELISA: Serpin E1/PAI-1 Antibody (1D5) - BSA Free [NBP2-37532]

ELISA: Serpin E1/PAI-1 Antibody (1D5) [NBP2-37532] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Western Blot: Serpin E1/PAI-1 Antibody (1D5) - BSA Free [NBP2-37532] -

Upregulation of ALDH1A3 in oxGBM cells is associated with the increased expression and release of pro-angiogenic factors PAI-1 and IL-8. GBM cell lines were transduced with ALDH1A3 for overexpression (oxGBM) or with empty vector (evGBM). (A) Confirmation of up-regulation of ALDH1A3 mRNA level in oxGBM cells by RT2-PCR. (B) Confirmation of up-regulation of ALDH1A3 protein expression by Western blot. wt, wild-type cells. The uncropped blots are shown in Figure S7; (C) Angiogenesis array. The blots showed duplicated dots for 55 angiogenesis-related proteins in the media of oxU373 or evU373. 10 of 55 proteins were upregulated more than 2-fold in ox group compared to ev group (indicated by rectangle). They are: (1) Ang-1, (2) artemin, (3) TF, (4) ET-1, (5) GM-CSF, (6) IL-8, (7) PDGF-AA, (8) PAI-1, (9) PEDF, and (10) uPA. (D) Semi-quantification of the dots representing PAI-1 and IL-8. (E) Immunofluorescence staining of GBM cells. U373 (left panel) and LN229 (right panel). Co-localization of ALDH1A3 with PAI-1 and IL-8 was observed in oxGBM cells, whereas no immunoreactivity of PAI-1 and IL-8 was detected in evGBM cells. (F) mRNA expression of PAI-1 and IL-8 in transduced U373 cells and the effect of inhibitors. Tiplaxtinin (Tip, 30 uM) and reparixin (Rep, 1 uM) are the specific inhibitors of PAI-1 and IL-8 receptors CXCR1/2, respectively. (G) Detection of PAI-1 and potential signaling proteins by Western blot. IOD: optical density. The uncropped blots are shown in Figure S8. **, p < 0.01; ***, p < 0.001, compared with ev. ##, p < 0.01; ###, p < 0.001, compared with ox. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37686698), licensed under a CC-BY license. Not internally tested by Novus Biologicals.