Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all sFRP-1 Primary Antibodies »

Product Specifications

Immunogen

sFRP-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein. (Uniprot: Q8N474)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with sFRP-1 from mouse, rat and bovine sources as only a single amino acid residue change is found within the immunogen sequence (91% positive by BLAST). Cross reactivity with sFRP-1 homologues from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity purified antibody is directed against human SFRP protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for sFRP-1 Antibody - BSA Free

Western Blot: sFRP-1 Antibody [NB600-499]

Western Blot: sFRP-1 Antibody [NB600-499]

Western Blot: sFRP-1 Antibody [NB600-499] - Analysis of a band 37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4C. Signal was detected using standard techniques. Personnel communication Becky Mercer, University of Columbia.
Immunohistochemistry: sFRP-1 Antibody [NB600-499]

Immunohistochemistry: sFRP-1 Antibody [NB600-499]

Immunohistochemistry: sFRP-1 Antibody [NB600-499] - Used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG is suitable for secondary antibody detection.
sFRP-1 Antibody

sFRP-1 Antibody

Affinity Purified anti-Human SFRP1 antibody was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG

Applications for sFRP-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:15000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

1:800

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:200-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, western blot, and IHC. This antibody is suitable for immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Sfrp1 by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: sFRP-1

SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Long Name

Secreted Frizzled Related Protein 1

Alternate Names

FrzA, SARP-2, sFRP1

Entrez Gene IDs

6422 (Human)

Gene Symbol

SFRP1

UniProt

Q8N474 (Human)

Additional sFRP-1 Products

Product Documents for sFRP-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for sFRP-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Family
Wnt Inhibitors

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for sFRP-1 Antibody - BSA Free

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  • Q: May I request confirmation that SFRP1 Antibody (NB600-499) has been tested in ELISA using cell lysate as sample instead of immunogen as sample? Will this antibody detect endogenous levels of the protein in ELISA? We have a customer who has had sensitivity issues with other SFRP1 antibodies and is looking for one that can detect endogenous levels of the protein. Thanks for your help. (This product lists ELISA as an application, what samples were used and can the endogenous protein be detected by ELISA?)

    A: I have pulled up information on this product and it looks like it has been tested in ELISA on the immunogen as opposed to an endogenous cell lysate. However, we do have a guarantee policy and we have confirmed this antibody to detect properly in Western Blot with cell lysate containing endogenous levels of the protein so it should also have an affinity for the protein in ELISA as well. (We have tested this SFRP1 antibody in ELISA using the native full length protein. While we have confirmed the presence of endogenous protein in cell lysate samples via western blot, we have not used cell lysate samples for detection in ELISA. Due to the western blot results, we predict that cell lysates containing endogenous levels of the SFRP1 protein should bind and detect the antibody.)

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