SFRS15 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88002

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SFRS15 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MHHQVPLPPNGQMPGFGLLPTPPFPPMAQPVIPPTPPVQQPFQASFQAQNEPLTQKPHQQEMEVEQPCIQEVKRHMSDNRKSRSRSASRSPK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SFRS15 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SFRS15 Antibody [NBP1-88002]

Immunocytochemistry/ Immunofluorescence: SFRS15 Antibody [NBP1-88002]

Immunocytochemistry/Immunofluorescence: SFRS15 Antibody [NBP1-88002] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002] - Staining of human cerebral cortex, kidney, skeletal muscle and testis using Anti-SCAF4 antibody NBP1-88002 (A) shows similar protein distribution across tissues to independent antibody NBP1-88001 (B).
Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002] - Staining in human testis and skeletal muscle tissues using anti-SCAF4 antibody. Corresponding SCAF4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002] - Staining of human kidney.
Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002]

Immunohistochemistry-Paraffin: SFRS15 Antibody [NBP1-88002] - Staining of human cerebral cortex.
Western Blot: SFRS15 Antibody [NBP1-88002]

Western Blot: SFRS15 Antibody [NBP1-88002]

Western Blot: SFRS15 Antibody [NBP1-88002] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Western Blot: SFRS15 Antibody [NBP1-88002]

Western Blot: SFRS15 Antibody [NBP1-88002]

Western Blot: SFRS15 Antibody [NBP1-88002] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-SCAF4 antibody. Remaining relative intensity is presented.

Applications for SFRS15 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SFRS15

The SFRS15 gene likely encodes a member of the arginine/serine-rich splicing factor family. A similar protein in Rat appearsto bind the large subunit of RNA polymerase II and provide a link between transcription and pre-mRNA splicing.Alternatively spliced trans

Alternate Names

CTD-binding SR-like protein RA4, FLJ23364, KIAA1172arginine/serine-rich 15, SFRS15, SR-like CTD-associated factor 4, SR-related and CTD-associated factor 4, SR-related CTD-associated factor 4

Gene Symbol

SCAF4

Additional SFRS15 Products

Product Documents for SFRS15 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SFRS15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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