SGK1 Antibody - BSA Free

Western Blot: SGK1 Antibody - BSA Free [NBP1-76578] -

Expression of mineralocorticoid receptor increases in rats 7 days after ischemia–reperfusion injury. Sprague Dawley rats underwent sham or ischemia–reperfusion injury (IRI) surgeries 14 days after right nephrectomy. IRI was induced by clamping the left renal artery. The rats were euthanized on day 7 after the operations, and kidney tissues were collected: (A) Western blots show expression levels of the mineralocorticoid receptor (MR), serum and glucocorticoid-regulated kinase 1 (SGK1), alpha -epithelial sodium channel ( alpha -ENaC), beta -ENaC, full-length gamma -ENaC, cleaved gamma -ENaC, phospho-NaCl cotransporter channel (Thr53) (p-NCCT53), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in kidneys from sham- and IRI-surgery rats. GAPDH was used as a loading control; (B) relative protein levels of MR, SGK1, alpha -ENaC, beta -ENaC, full-length gamma -ENaC, cleaved gamma -ENaC, and p-NCCT53 in the sham and IRI groups. Band intensities were normalized to GAPDH; (C) plasma aldosterone concentrations in the sham and IRI groups. Values are presented as mean +/- standard error (n = 5 rats per group). Data were analyzed by Student’s t-test for comparisons between two groups. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35887178), licensed under a CC-BY license. Not internally tested by Novus Biologicals.