SH3BP5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80796

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SH3BP5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ETQSVSSFSSGPTSPSEMPDQFPAVVRPGSLDLPSPVSLSEFGMMFPVLGPRSECSGASSPECEVERGDRAEGAENKTSDKANNNRG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SH3BP5 Antibody - BSA Free

Western Blot: SH3BP5 Antibody [NBP1-80796]

Western Blot: SH3BP5 Antibody [NBP1-80796]

Western Blot: SH3BP5 Antibody [NBP1-80796] - Analysis in human cell lines MCF-7 and Caco-2 using anti-SH3BP5 antibody. Corresponding SH3BP5 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: SH3BP5 Antibody [NBP1-80796]

Immunocytochemistry/ Immunofluorescence: SH3BP5 Antibody [NBP1-80796]

Immunocytochemistry/Immunofluorescence: SH3BP5 Antibody [NBP1-80796] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796]

Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796]

Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796] - Staining of human Prostate shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796]

Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796]

Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796] - Staining of human Kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796]

Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796]

Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796] - Staining of human Pancreas shows very weak granular cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796]

Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796]

Immunohistochemistry-Paraffin: SH3BP5 Antibody [NBP1-80796] - Staining of human Testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.

Applications for SH3BP5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SH3BP5

SH3BP5 (SH3 domain binding protein 5) binds to the SH3 domain of Bruton tyrosine kinase (BTK), a cytoplasmic tyrosine kinase crucial for the maturation of B-lineage cells. It inhibits the auto- and transphosphorylation activity of BTK and negatively regulates BTK-related cytoplasmic signaling in B-cells. SH3BP5 also interacts with JNK. The interaction with JNK occurs through a mitogen-activated protein KIM (kinase interaction motif) in a region distinct from the Bruton's tyrosine kinase-binding domain.

Alternate Names

Sab, SABSH3BP-5, SH3 binding protein, SH3 domain-binding protein 5, SH3 domain-binding protein that preferentially associates with BTK, SH3-domain binding protein 5 (BTK-associated)

Entrez Gene IDs

9467 (Human)

Gene Symbol

SH3BP5

UniProt

O60239 (Human)

Additional SH3BP5 Products

Product Documents for SH3BP5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SH3BP5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SH3BP5 Antibody - BSA Free

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Protocols

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FAQs

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