SHC1 [p Tyr427] Antibody - Azide and BSA Free

Name: SHC1 [p Tyr427] Antibody - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-16938
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16938

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

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Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y427 of human SHC1 (NP_892113.4). PSYVN

Modification

p Tyr 427

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHC1 [p Tyr427] Antibody - Azide and BSA Free

Western Blot: SHC1 [p Tyr427] AntibodyAzide and BSA Free [NBP3-16938]

Western Blot: SHC1 [p Tyr427] Antibody [NBP3-16938] - Western blot analysis of extracts of A-431 cells, using SHC1 antibody (NBP3-16938) at 1:1000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for SHC1 [p Tyr427] Antibody - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHC1

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Gene Symbol

SHC1

Additional SHC1 Products

Product Documents for SHC1 [p Tyr427] Antibody - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for SHC1 [p Tyr427] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov