SHISA3 Antibody - BSA Free

1 out of 5

1 Customer Rating

5 Stars

0%

4 Stars

0%

3 Stars

0%

2 Stars

0%

1 Stars

100%

Have you used SHISA3 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Customer Images

• 
• (1)

• SHISA3 Antibody

  Name: Anonymous

  Application: Western Blot

  Sample Tested: Bone marrow-derived macrophages

  Species: Mouse

  Verified Customer | Posted 12/07/2021

  Western blot analysis of wildtype and Mkp-1-/- BMDM treated with LPS for different times.

  Mouse bone-marrow-derived macrophages were stimulated with 100 ng/ml for 2, 3, 4, 6, 8, 12, 16, 20, and 24 h or left unstimulated as control, label as 0 h; in DMEM+10% FBS, and lysed in lysis buffer (20 mM HEPES, pH7.4, 50 mM beta-glycerol phosphate, 2 mM EGTA, 1 mM DTT, 10 mM NaF, 1 mM Sodium Orthovanadate, 1% Triton-100, 10% Glycerol, and various protease and phosphatase inhibitors). Lysates were resoled on 4-12% NuPAGE gel under denature condition. After transfering to nitrocellulose membrane, the blot was blocked in TBST containing 5% (w/v) Blotting Grade Blocker Non Fat Dry Milk (Bio-Rad) overnight at 4C. After blocking, the membrane was incubated with the primary antibody diluted to 1 ug/ml in TBST containing 5% BSA and 0.02% sodium azide for 1 h at room temperature or 60 h at 4 C. After 3 washes with TBST (5', 10' and 15') the blot was incubated with HRP-conjugated goat anti-rabbit secondary antibody (1:15,000) for 1 h at room temperature. After washing 3 times with TBST, the image was developed using ECL.

  
  Bio-Techne Response

  Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

There are no reviews that match your criteria.