SHMT2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-80754
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0020.jpg "Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Cytometric Bead Assay Standard, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAF
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25855294).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit SHMT2 Antibody - BSA Free (NBP1-80754) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-SHMT2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SHMT2 Antibody - BSA Free
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0027.jpg "Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]")
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human duodenum, liver, lymph node and skeletal muscle using Anti-SHMT2 antibody NBP1-80754 (A) shows similar protein distribution across tissues to independent antibody NBP1-80755 (B).![Western Blot: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Western-Blot-NBP1-80754-img0022.jpg "Western Blot: SHMT2 Antibody [NBP1-80754]")
![Immunocytochemistry/ Immunofluorescence: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80754-img0009.jpg "Immunocytochemistry/ Immunofluorescence: SHMT2 Antibody [NBP1-80754]")
Immunocytochemistry/ Immunofluorescence: SHMT2 Antibody [NBP1-80754]
Immunocytochemistry/Immunofluorescence: SHMT2 Antibody [NBP1-80754] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0028.jpg "Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]")
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.![Western Blot: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Western-Blot-NBP1-80754-img0007.jpg "Western Blot: SHMT2 Antibody [NBP1-80754]")
Western Blot: SHMT2 Antibody [NBP1-80754]
Western Blot: SHMT2 Antibody [NBP1-80754] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).![Western Blot: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Western-Blot-NBP1-80754-img0010.jpg "Western Blot: SHMT2 Antibody [NBP1-80754]")
Western Blot: SHMT2 Antibody [NBP1-80754]
Western Blot: SHMT2 Antibody [NBP1-80754] - Analysis in human cell line RH-30.![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0016.jpg "Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]")
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0017.jpg "Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]")
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human lymph node shows moderate granular cytoplasmic positivity in germinal center cells.![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]](https://resources.rndsystems.com/images/products/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0018.jpg "Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]")
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754]
Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human skeletal muscle shows no positivity in myocytes as expected.Applications for SHMT2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 25855294). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SHMT2
Long Name
Serine hydroxymethyltransferase, mitochondrial
Alternate Names
EC 2.1.2.1, GLY A+, GLYA, glycine auxotroph A, human complement for hamster, Glycine hydroxymethyltransferase, serine aldolase, serine hydroxymethylase, serine hydroxymethyltransferase 2 (mitochondrial), serine hydroxymethyltransferase, mitochondrial, Serine methylase, SHMT, threonine aldolase
Entrez Gene IDs
6472 (Human)
Gene Symbol
SHMT2
UniProt
Additional SHMT2 Products
Product Documents for SHMT2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SHMT2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SHMT2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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