Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Novus Biologicals | Catalog # NB600-534

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Virus

Cited:

Human, Mouse, Porcine, Virus

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay, Radioimmunoassay, In vivo assay

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HSn 7D2

Format

BSA Free
View all Siglec-1/CD169 Primary Antibodies »

Product Specifications

Immunogen

Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]

Epitope

Within domains 1-4.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27441395). Mouse reactivity reported in scientific literature. Not yet tested in other species. Use in Virus reported in scientific literature (PMID:27487279).

Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.
Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).
Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.

Applications for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 28794041)

Immunohistochemistry

1:10-1:500. Use reported in scientific literature (PMID 26106049)

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

In vivo assay

reported in scientific literature (PMID 18414664)

Western Blot

reported in scientific literature
Application Notes
This Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA.

Reviewed Applications

Read 1 review rated 5 using NB600-534 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-1/CD169

Sialoadhesin, also known as sialic acid-binding Ig-like lectin 1, siglec-1, CD169, SIGLEC1, and SN, is a member of the sialic acid binding Ig-like lectin (SIGLEC) family. The SIGLECs are a family of membrane bound lectins that belong to the immunoglobulin superfamily. SIGLECs bind sialic acid and mediate cell to cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a lectin-like macrophage-specific cell adhesion molecule that mediates sialic acid dependent binding of glycoconjugate ligands on activated macrophages, lymphocytes, granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. The Sialoadhesin protein is found in two locations within the cell, the cell membrane (isoform 1) and secreted in the cytoplasm (isoform 2). Sialoadhesin is expressed in spleen, lymph node, brain, bone marrow, liver, colon and lung tissues and in inflammatory macrophages in rheumatoid arthritis.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Entrez Gene IDs

6614 (Human)

Gene Symbol

SIGLEC1

UniProt

Q9BZZ2 (Human)

Additional Siglec-1/CD169 Products

Product Documents for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Customer Reviews for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free (1)

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Customer Images

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  • Siglec-1/CD169 Antibody (HSn 7D2)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult small intestine
    Species: Mouse
    Verified Customer | Posted 12/16/2018
    NB600-534 Siglec-1 was used at 1:500 and labelled with Alexa Fluor 568 conjugated secondary antibody - red. DAPI shown as blue.
    Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free NB600-534

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Protocols

View specific protocols for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free (NB600-534):


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

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