Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-54473

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BLCAM/1795

Format

Azide and BSA Free
View all Siglec-2/CD22 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa52-178) of human Siglec-2/CD22 protein (exact sequence is proprietary) (Uniprot: P20273)

Localization

Cell Surface

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53239).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free

Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]

Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]

Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).
Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Formalin-fixed, paraffin-embedded human Spleen stained with Siglec-2/CD22 Antibody (BLCAM/1795).
Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Flow Cytometric Analysis of paraformaldehyde-fixed MOLT4 cells. Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]

Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]

Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Analysis of Raji and Ramos Cell Lysates using CD22 Monoclonal Antibody (BLCAM/1795).
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD22 Monoclonal Antibody (BLCAM/1795).
Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]

Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Siglec-2/CD22 Antibody (BLCAM/1795) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free

Application
Recommended Usage

ELISA

2 - 4 ug/ml

Flow Cytometry

0.5 - 1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5 - 1 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Western Blot

0.5 - 1.0 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Siglec-2/CD22

CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Gene Symbol

CD22

Additional Siglec-2/CD22 Products

Product Documents for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free

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  • Q: I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?

    A: We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

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