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Simple Plex HIV-1 Gag p24 Cartridge

Catalog #: SPCKB-PS-000981 Datasheet / COA / SDS
Simple Plex Human HIV-1 Gag p24 assay kit for use on Ella automated benchtop ELISA. Contains cartridge, sample diluent SD30, and wash buffer
Catalog # Availability Size / Price Qty
SPCKA-PS-000981
SPCKC-PS-000981
SPCKB-PS-000981
ST01B-PS-000981
HIV Gag p24 Simple Plex Assay
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex HIV-1 Gag p24 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
3.93 pg/mL
Assay Range
3.9 - 15,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant HIV-1 Gag p24.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of HIV Gag p24 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) on Ella automated benchtop ELISA. 

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 105 4500 94.3 4074
Standard Deviation 0.37 237 9.64 377
CV% 7.1 5.3 10.2 9.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 114 111-118
EDTA Plasma (n=4) 107 102-116
Heparin Plasma (n=4) 107 99-112
Serum (n=4) 111 109-113

Linearity

Samples containing and/or spiked with high concentrations of human HIV Gag p24 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
HIV Gag p24 Simple Plex Assay Linearity

Scientific Data

N/A View Larger

HIV Gag p24 Simple Plex Assay Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name:
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs:
155030 (Viral)
Alternate Names:
CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag)

FAQs

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