Simple Plex HIV-1 Gag p24 Cartridge
Simple Plex HIV-1 Gag p24 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
Recovery at three different spiked concentrations within the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Supernates (n=4)||114||111-118|
|EDTA Plasma (n=4)||107||102-116|
|Heparin Plasma (n=4)||107||99-112|
HIV Gag p24 Simple Plex Assay Standard Curve
Preparation and Storage
Background: HIV-1 Gag p24
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
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