Simple Plex Human NF-H Cartridge

Simple Plex Human NF-H assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer
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Human NF-H Simple Plex Assay
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Product Details
Citations (4)
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Simple Plex Human NF-H Cartridge Summary

1.17 pg/mL
Assay Range
7.5 - 28,480 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Natural and recombinant human NF-H.
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human NF-H in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF)


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 10 9
Mean (pg/mL) 43.4 2206 47.4 2881
Standard Deviation 2.22 81.6 6.59 365
CV% 5.1 3.7 13.9 12.7


Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=3) 98 93-101
EDTA Plasma (n=4) 94 83-108
Heparin Plasma (n=4) 102 99-107
Serum (n=4) 102 82-114


Samples containing and/or spiked with high concentrations of human NF-H were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human NF-H Simple Plex Assay Linearity

Scientific Data

Human NF-H Simple Plex Assay Standard Curve

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NF-H

Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.

Long Name:
Neurofilament Protein, Heavy Chain
Entrez Gene IDs:
4744 (Human)
Alternate Names:
200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H

Citations for Simple Plex Human NF-H Cartridge

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Neurofilament as a potential biomarker for spinal muscular atrophy.
    Authors: Darras B, Crawford T, Finkel R, Mercuri E, De Vivo D, Oskoui M, Tizzano E, Ryan M, Muntoni F, Zhao G, Staropoli J, McCampbell A, Petrillo M, Stebbins C, Fradette S, Farwell W, Sumner C
    Ann Clin Transl Neurol, 0;6(5):932-944.
    Species: Human
    Sample Types: Plasma
  2. Plasma Neurofilament Light for Prediction of Disease Progression in Familial Frontotemporal Lobar Degeneration.
    Authors: Rojas J, Wang P, Staffaroni A, Heller C, Cobigo Y, Wolf A, Goh S, Ljubenkov P, Heuer H, Fong J, Taylor J, Veras E, Song L, Jeromin A, Hanlon D, Yu L, Khinikar A, Sivasankaran R, Kieloch A, Valentin M, Karydas A, Mitic L, Pearlman R, Kornak J, Kramer J, Miller B, Kantarci K, Knopman D, Graff-Radford N, Petrucelli L, Rademakers R, Irwin D, Grossman M, Ramos E, Coppola G, Mendez M, Bordelon Y, Dickerson B, Ghoshal N, Huey E, Mackenzie I, Appleby B, Domoto-Reilly K, Hsiung G, Toga A, Weintraub S, Kaufer D, Kerwin D, Litvan I, Onyike C, Pantelyat A, Roberson E, Tartaglia M, Foroud T, Chen W, Czerkowicz J, Graham D, van Swieten J, Borroni B, Sanchez-Valle R, Moreno F, Laforce R, Graff C, Synofzik M, Galimberti D, Rowe J, Masellis M, Finger E, Vandenberghe R, de Mendonca A, Tagliavini F, Santana I, Ducharme S, Butler C, Gerhard A, Levin J, Danek A, Otto M, Sorbi S, Cash D, Convery R, Bocchetta M, Foiani M, Greaves C, Peakman G, Russell L, Swift I, Todd E, Rohrer J, Boeve B, Rosen H, Boxer A
    Neurology, 0;96(18):e2296-e2312.
    Species: Human
    Sample Types: CSF
  3. Implications of circulating neurofilamentsfor spinal muscular atrophytreatment early in life: A case series.
    Authors: Alves C, Petrillo M, Spellman R, Garner R, Zhang R, Kiefer M, Simeone S, Sohn J, Eichelberger E, Rodrigues E, Arruda E, Townsend E, Farwell W, Swoboda K
    Mol Ther Methods Clin Dev, 0;23(0):524-538.
    Species: Human
    Sample Types: Serum
  4. Comparison of CSF and serum neurofilament light and heavy chain as differential diagnostic biomarkers for ALS.
    Authors: Halbgebauer S, Steinacker P, Verde F, Weishaupt J, Oeckl P, von Arnim C, Dorst J, Feneberg E, Mayer B, Rosenbohm A, Silani V, Ludolph A, Otto M
    Oncogene, 0;93(1):68-74.
    Species: Human
    Sample Types: CSF


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