Simple Plex Human NF-L Cartridge

Simple Plex Cartridge Kit for quantitation of NF-L. Includes wash buffer and sample diluent SD13. For use with the Ella automated immunoassay system
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Control Products Available
Human NF-L Ella Assay
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Product Details
Citations (11)
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Simple Plex Human NF-L Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
1.09 pg/mL
Assay Range
2.7 - 10,290 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Natural and recombinant NF-L.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Summary

Simple Plex assay for the detection of human Neurofilament Light (NF-L) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF). Simple Plex assays on Ella offer precision multiplexing in a simple format. The preloaded standard curves and hand-free automation reduce human error and variability. Every assay on the Ella menu is highly sensitive and fully validated.


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 48 48
Mean (pg/mL) 76.6 3309 80.5 3476
Standard Deviation 3.64 154 8.35 267
CV% 4.8 4.7 10.4 7.7


Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 112 110-115
EDTA Plasma (n=4) 93 86-99
Heparin Plasma (n=4) 101 93-106
Serum (n=4) 108 95-115


Samples containing and/or spiked with high concentrations of NF-L were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Human NF-L Ella Assay Linearity

Scientific Data

Human NF-L Ella Assay Standard Curve

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NF-L

Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.

Long Name:
Neurofilament Protein, Light Chain
Entrez Gene IDs:
4747 (Human)
Alternate Names:
68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa

Citations for Simple Plex Human NF-L Cartridge

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Neurochemical Monitoring of Traumatic Brain Injury by the Combined Analysis of Plasma Beta-Synuclein, NfL, and GFAP in Polytraumatized Patients
    Authors: R Halbgebaue, S Halbgebaue, P Oeckl, P Steinacker, E Weihe, MK Schafer, F Roselli, F Gebhard, M Huber-Lang, M Otto
    International Journal of Molecular Sciences, 2022;23(17):.
    Species: Human
    Sample Types: Plasma
  2. The "develOpment of metabolic and functional markers of Dementia IN Older people" (ODINO) Study: Rationale, Design and Methods
    Authors: A Picca, D Ronconi, HJ Coelho-Jun, R Calvani, F Marini, A Biancolill, J Gervasoni, A Primiano, C Pais, E Meloni, D Fusco, MR Lo Monaco, R Bernabei, MC Cipriani, E Marzetti, R Liperoti
    J Pers Med, 2020;10(2):.
    Species: Human
    Sample Types: Serum
  3. Serum neurofilament light as biomarker of seizure-related neuronal injury in status epilepticus.
    Authors: Giovannini G, Bedin R, Ferraro D, Vaudano A, Mandrioli J, Meletti S
    Epilepsia, 0;63(1):e23-e29.
    Species: Human
    Sample Types: Serum
  4. Comparison of Simoa(TM) and Ella(TM) to assess serum neurofilament-light chain in multiple sclerosis.
    Authors: Gauthier A, Viel S, Perret M, Brocard G, Casey R, Lombard C, Laurent-Chabalier S, Debouverie M, Edan G, Vukusic S, Lebrun-Frenay C, De Seze J, Laplaud D, Castelnovo G, Gout O, Ruet A, Moreau T, Casez O, Clavelou P, Berger E, Zephir H, Trouillet-Assant S, Thouvenot E
    Ann Clin Transl Neurol, 0;8(5):1141-1150.
    Species: Human
    Sample Types: Serum
  5. Serum neurofilament light chain and initial severity of neurological disease predict the early neurological deterioration in Wilson's disease.
    Authors: Ziemssen T, Smolinski L, Czlonkowska A, Akgun K, Antos A, Bembenek J, Kurkowska-Jastrzebska I, Przybylkowski A, Skowronska M, Redzia-Ogrodnik B, Litwin T
    Acta Neurol Belg, 0;0(0):1-9.
    Species: Human
    Sample Types: Plasma
  6. White matter alterations in chronic MDMA use: Evidence from diffusion tensor imaging and neurofilament light chain blood levels.
    Authors: Zimmermann J, Friedli N, Bavato F, Stampfli P, Coray R, Baumgartner M, Grandgirard D, Leib S, Opitz A, Seifritz E, Stock A, Beste C, Cole D, Quednow B
    Neuroimage Clin, 0;36(0):103191.
    Species: Human
    Sample Types: Serum
  7. Association of admission serum levels of neurofilament light chain and in-hospital mortality in geriatric patients with COVID-19.
    Authors: Marchegiani F, Recchioni R, Marcheselli F, Di Rosa M, Sabbatinelli J, Matacchione G, Giuliani A, Ramini D, Stripoli P, Biscetti L, Pelliccioni G, Sarzani R, Spannella F, Cherubini A, Corsonello A, Procopio A, Bonfigli A, Bonafe M, Lattanzio F, Olivieri F
    Oncogene, 0;0(0):1-7.
    Species: Human
    Sample Types: Serum
  8. Serum phosphorylated tau protein 181 and neurofilament light chain in cognitively impaired heart failure patients.
    Authors: Traub J, Otto M, Sell R, Gopfert D, Homola G, Steinacker P, Oeckl P, Morbach C, Frantz S, Pham M, Stork S, Stoll G, Frey A
    Alzheimers Res Ther, 0;14(1):149.
    Species: Human
    Sample Types: Serum
  9. Glial fibrillary acidic protein in cerebrospinal fluid of patients with spinal muscular atrophy.
    Authors: Freigang M, Steinacker P, Wurster C, Schreiber-Katz O, Osmanovic A, Petri S, Koch J, Rostasy K, Huss A, Tumani H, Winter B, Falkenburger B, Ludolph A, Otto M, Hermann A, Gunther R
    Ann Clin Transl Neurol, 0;9(9):1437-1448.
    Species: Human
    Sample Types: CSF
  10. Comparison of CSF and serum neurofilament light and heavy chain as differential diagnostic biomarkers for ALS.
    Authors: Halbgebauer S, Steinacker P, Verde F, Weishaupt J, Oeckl P, von Arnim C, Dorst J, Feneberg E, Mayer B, Rosenbohm A, Silani V, Ludolph A, Otto M
    Oncogene, 0;93(1):68-74.
    Species: Human
    Sample Types: CSF
  11. CSF Biomarkers in COVID-19 Associated Encephalopathy and Encephalitis Predict Long-Term Outcome.
    Authors: Guasp M, Munoz-Sanchez G, Martinez-Hernandez E, Santana D, Carbayo A, Naranjo L, Bolos U, Framil M, Saiz A, Balasa M, Ruiz-Garcia R, Sanchez-Valle R
    Front Immunol, 0;13(0):866153.
    Species: Human
    Sample Types: CSF


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