Simple Plex Human Prolactin Cartridge

Simple Plex Cartridge Kit for 72 samples, containing Prolactin for use with Human Plasma/Serum. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

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Simple Plex Human Prolactin Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)
Sensitivity
104 pg/mL
Assay Range
104.0 - 45,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Prolactin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Prolactin in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assa
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean 346 16436 329 15289
Standard Deviation 10.2 515 31 996
CV% 3 3.1 9.4 6.5

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 100 92-109
Heparin Plasma (n=4) 98 96-101
Serum (n=4) 96 90-106

Linearity

Samples containing and/or spiked with high concentrations of human Prolactin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Prolactin

Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.

Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.

Entrez Gene IDs:
5617 (Human); 19109 (Mouse)
Alternate Names:
PRL; Prolactin

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