SIRP alpha/CD172a Antibody (BLR049F)

Name: SIRP alpha/CD172a Antibody (BLR049F)
Brand: Novus Biologicals
SKU: NBP3-14675
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14675

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Product Documents

Recombinant Monoclonal

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR049F

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Product Specifications

Immunogen

residues 31-368 (ECD)

Clonality

Monoclonal

Host

Rabbit

Description

Novus Biologicals Rabbit SIRP alpha/CD172a Antibody (BLR049F) (NBP3-14675) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SIRP alpha/CD172a Antibody (BLR049F)

Western Blot: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Western Blot: SIRP alpha/CD172a Antibody [NBP3-14675] - Whole cell lysate (50 ug) from Malme-3M, HeLa, 786-O, HEK293T, U2OS, OVCAR-4, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiActin.

Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Immunocytochemistry/Immunofluorescence: SIRP alpha/CD172a Antibody [NBP3-14675] - Malme-3M cells. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP3-14675] - Human breast carcinoma. Antibody: Rabbit anti-SIRP alpha recombinant monoclonalantibody [BLR049F] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.

Immunoprecipitation: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Immunoprecipitation: SIRP alpha/CD172a Antibody [NBP3-14675] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-MEL-28 cells prepared using NETN lysis buffer. Antibodies: Rabbit antiSIRP alpha recombinant monoclonal antibody used for IP at 20 ul per reaction. SIRP alpha was also immunoprecipitated by rabbit anti-SIRP alpha recombinant monoclonal antibody. For blotting immunoprecipitated SIRP alpha, was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

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Applications for SIRP alpha/CD172a Antibody (BLR049F)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100- 1:500

Immunohistochemistry-Paraffin

1:100- 1:500

Immunoprecipitation

20 ul/1 mg lysate

Western Blot

1:1000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

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Product Specific Notices for SIRP alpha/CD172a Antibody (BLR049F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.