SIRP alpha/CD172a Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-56723

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SIRP alpha/CD172a Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: RIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQAAEPN

Reactivity Notes

Mouse 81%, Rat 80%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SIRP alpha/CD172a Antibody - BSA Free (NBP2-56723) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SIRP alpha/CD172a Antibody - BSA Free

Western Blot: SIRP alpha/CD172a Antibody [NBP2-56723]

Western Blot: SIRP alpha/CD172a Antibody [NBP2-56723]

Western Blot: SIRP alpha/CD172a Antibody [NBP2-56723] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer, neuropil was distinctly stained.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human liver.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human colon.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human kidney.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human cerebellum, cerebral cortex, pancreas and skeletal muscle using Anti-SIRPA antibody NBP2-56723 (A) shows similar protein distribution across tissues to independent antibody NBP2-58429 (B).
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-56723 antibody. Corresponding SIRPA RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in granular layer.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for SIRP alpha/CD172a Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SIRP alpha/CD172a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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