Sirtuin 3/SIRT3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-02984
![Knockout Validated: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]](https://resources.rndsystems.com/images/products/Sirtuin-3-SIRT3-Antibody-Knockout-Validated-NBP3-02984-img0008.jpg "Western Blot: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 299-399 of human Sirtuin 3/SIRT3 (NP_036371.1). PQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Sirtuin 3/SIRT3 Antibody - BSA Free
Western Blot: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]
Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts from wild type(WT) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]](https://resources.rndsystems.com/images/products/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP3-02984-img0007.jpg "Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]")
Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]
Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [Sirtuin 3/SIRT3] -
Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [Sirtuin 3/SIRT3] - Immunofluorescence analysis of NIH/3T3 cells using Sirtuin 3/SIRT3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]](https://resources.rndsystems.com/images/products/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0003.jpg "Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]")
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Mouse kidney using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]](https://resources.rndsystems.com/images/products/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP3-02984-img0006.jpg "Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]")
Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]
Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of Mouse liver, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]](https://resources.rndsystems.com/images/products/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0001.jpg "Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]")
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Human thyroid cancer using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]](https://resources.rndsystems.com/images/products/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0002.jpg "Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]")
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Rat kidney using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Sirtuin 3/SIRT3 Antibody - BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:20 - 1:50
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Sirtuin 3/SIRT3
Long Name
Silent Mating Type Information Regulation 3 Homolog
Alternate Names
SIR2-like protein 3, SIR2L3, SIRT3
Gene Symbol
SIRT3
Additional Sirtuin 3/SIRT3 Products
Product Documents for Sirtuin 3/SIRT3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Sirtuin 3/SIRT3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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