Sirtuin 5/SIRT5 Antibody (OTI2F1)
Novus Biologicals | Catalog # NBP2-01003
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2F1
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Product Specifications
Immunogen
Full length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Sirtuin 5/SIRT5 Antibody (OTI2F1)
![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.rndsystems.com/images/products/Sirtuin-5-SIRT5-Antibody-OTI2F1-Western-Blot-NBP2-01003-img0017.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.![Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.rndsystems.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Immunocytochemistry-Immunofluorescence-NBP2-01003-img0013.jpg "Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Immunocytochemistry/Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.rndsystems.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Immunohistochemistry-Paraffin-NBP2-01003-img0014.jpg "Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody.![Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.rndsystems.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Flow-Cytometry-NBP2-01003-img0012.jpg "Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Flow Cytometry: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody, and then analyzed by flow cytometry.![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.rndsystems.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Western-Blot-NBP2-01003-img0015.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Western Blot: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.rndsystems.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Western-Blot-NBP2-01003-img0011.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.Applications for Sirtuin 5/SIRT5 Antibody (OTI2F1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.24 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Sirtuin 5/SIRT5
Long Name
Silent Mating Type Information Regulation 5 Homolog
Alternate Names
SIR2L5, SIRT5
Entrez Gene IDs
23408 (Human)
Gene Symbol
SIRT5
Additional Sirtuin 5/SIRT5 Products
Product Documents for Sirtuin 5/SIRT5 Antibody (OTI2F1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Sirtuin 5/SIRT5 Antibody (OTI2F1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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