Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74199

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F1

Format

Azide and BSA Free
View all Sirtuin 5/SIRT5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1)Azide and BSA Free [NBP2-74199]

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1)Azide and BSA Free [NBP2-74199]

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199]

Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199]

Immunocytochemistry/Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.
Immunohistochemistry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199]

Immunohistochemistry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199]

Immunohistochemistry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody.
Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199]

Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199]

Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SIRT5 antibody, and then analyzed by flow cytometry.
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1)Azide and BSA Free [NBP2-74199]

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1)Azide and BSA Free [NBP2-74199]

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1)Azide and BSA Free [NBP2-74199]

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1)Azide and BSA Free [NBP2-74199]

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with ant

Applications for Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Sirtuin 5/SIRT5

SIRT5 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.

Long Name

Silent Mating Type Information Regulation 5 Homolog

Alternate Names

SIR2L5, SIRT5

Gene Symbol

SIRT5

Additional Sirtuin 5/SIRT5 Products

Product Documents for Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Customer Reviews for Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free and earn rewards!

Have you used Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies