SLC25A13 Antibody - BSA Free

Western Blot: SLC25A13 Antibody [NBP1-33380]

Western Blot: SLC25A13 Antibody [NBP1-33380] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal diluted at 1:500.

Western Blot: SLC25A13 Antibody [NBP1-33380]

Western Blot: SLC25A13 Antibody [NBP1-33380] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: SLC25A13 Antibody [NBP1-33380]

Immunohistochemistry-Paraffin: SLC25A13 Antibody [NBP1-33380] - Paraffin-embedded N87 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: SLC25A13 Antibody [NBP1-33380]

Immunohistochemistry-Paraffin: SLC25A13 Antibody [NBP1-33380] - Paraffin-embedded mouse kidney. SLC25A13 antibody [N3C2], Internal diluted at 1:500.

Western Blot: SLC25A13 Antibody [NBP1-33380] -

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with SLC25A13 antibody [N3C2], Internal (NBP1-33380) diluted at 1:1000 and competitor's antibody (10789-1-AP) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

*The competitor is not affiliated with Novus and does not endorse this product.

Immunocytochemistry/ Immunofluorescence: SLC25A13 Antibody [NBP1-33380] -

SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLC25A13 protein stained by SLC25A13 antibody [N3C2], Internal (NBP1-33380) diluted at 1:500.
Blue: Hoechst 33342 staining.

Western Blot: SLC25A13 Antibody - BSA Free [NBP1-33380] -

TNF impairs the malate aspartate shuttle leading to a NAD+ and NADH deficit. (A) Experimental setup for liver genome-wide transcriptomic analysis 18h after TNF (25 ug). (B) Enrichr pathway analysis (Elsevier pathway collection) of the downregulated genes 18h after TNF (1310 genes) with LFC < -1 and p < 0.05. (C) Schematic overview of the MAS and metabolic pathways depending on NAD+/NADH homeostasis. 1: GOT1; 2: MDH1; 3. OGC; 4: MDH2; 5: GOT2; 6: AGC; ETC: electron transport chain; OXPHOS: oxidative phosphorylation; alpha KG: alpha ketoglutarate. (D, E). Enrichr pathway analysis (Metabolomics Workbench metabolites 2022 (D) and GO cellular component 2023 (E) of the downregulated genes 18h after TNF (1310 genes) with LFC < -1 and p < 0.05. (F) Heatmap depicting the relative expression levels (%) of genes involved in the MAS 18h after TNF, with RNASeq mRNA counts from the PBS condition set at 100%. (G) mRNA counts of Slc25a13 in livers of PBS- and TNF-treated mice. n=3/group. (H, I). Western blot analysis of SLC25A13 (74 kDa) protein levels in livers of PBS- and TNF-treated mice (35 ug), normalized to actin levels (42 kDa). n=3-5/group. (J, K). Liver NAD+(J) and NADH (K) levels in PBS- and TNF-injected mice (35 ug), relative to PBS controls. n=3-5/group. (L). Enrichr pathway analysis (TF-LOF expression from GEO) of the downregulated genes 18h after TNF (1310 genes) with LFC < -1 and p < 0.05. (M). HOMER motif analysis of the 1310 downregulated genes. (N). Hnf4 alpha Liver-i-KO and Hnf4 alpha fl/fl were IP injected with TNF (32,5 ug) and mortality was monitored. n=3-6/group. Bars: mean +/- SEM. Each dot represents a single biological replicate. P-values were analyzed with unpaired t-test (G, H, J, K). Survival was analyzed via a log rank test (N). **p ≤ 0.01, *p ≤ 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41132685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.