SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80954
![Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]](https://resources.rndsystems.com/images/products/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Western-Blot-NBP2-80954-img0002.jpg "Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse, Rat, Human (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # mAb16
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977]
Epitope
N-terminus
Reactivity Notes
Little to no human reactivity has been observed.
Localization
Membrane; Multi-pass membrane protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]
Western Blot: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]](https://resources.rndsystems.com/images/products/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80954-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]")
Immunocytochemistry/ Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]
Immunocytochemistry/Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.![Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]](https://resources.rndsystems.com/images/products/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80954-img0004.jpg "Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]")
Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]
Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.![Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]](https://resources.rndsystems.com/images/products/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Western-Blot-NBP2-80954-img0001.jpg "Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]")
Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]
Western Blot: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - Analysis of DAT1 expression in rat striatal membrane tissue lysate. Image from the standard format of this antibody.Applications for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
reported in scientific literature (PMID 20643191)
Western Blot
1:1000
Application Notes
In WB a band can been seen at ~70-85 kDa.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLC6A3/DAT1
Long Name
Dopamine Transporter
Alternate Names
DAT1, SLC6A3, VNTR
Gene Symbol
SLC6A3
Additional SLC6A3/DAT1 Products
Product Documents for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free and earn rewards!
Have you used SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
A: Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.
Loading...