SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80954
Key Product Details
Species Reactivity
Mouse, Rat, Human (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # mAb16
Format
Azide and BSA Free
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Product Specifications
Immunogen
Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977]
Epitope
N-terminus
Reactivity Notes
Little to no human reactivity has been observed.
Localization
Membrane; Multi-pass membrane protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
![Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]](https://resources.rndsystems.com/images/products/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Western-Blot-NBP2-80954-img0002.jpg "Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]")
Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]
Western Blot: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]](https://resources.rndsystems.com/images/products/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80954-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]")
Immunocytochemistry/ Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]
Immunocytochemistry/Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.![Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]](https://resources.rndsystems.com/images/products/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80954-img0004.jpg "Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]")
Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954]
Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.![Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]](https://resources.rndsystems.com/images/products/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Western-Blot-NBP2-80954-img0001.jpg "Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]")
Western Blot: SLC6A3/DAT1 Antibody (mAb16)Azide and BSA Free [NBP2-80954]
Western Blot: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - Analysis of DAT1 expression in rat striatal membrane tissue lysate. Image from the standard format of this antibody.Applications for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
reported in scientific literature (PMID 20643191)
Western Blot
1:1000
Application Notes
In WB a band can been seen at ~70-85 kDa.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLC6A3/DAT1
Long Name
Dopamine Transporter
Alternate Names
DAT1, SLC6A3, VNTR
Gene Symbol
SLC6A3
Additional SLC6A3/DAT1 Products
Product Documents for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free
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Q: Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
A: Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.
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