SLFN5 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-81178
![Western Blot: SLFN5 Antibody [NBP1-81178]](https://resources.rndsystems.com/images/products/SLFN5-Antibody-Western-Blot-NBP1-81178-img0012.jpg "Western Blot: SLFN5 Antibody [NBP1-81178]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ERHGVGLDVPPIFRSHLDKMQKENHFLIFVKSWNTEAGVPLATLCSNLYHRERTSTDVMDSQEALAFLKCRTQTPTNINVSNSLGPQAAQGSVQYEGNINVSAAALFDRKRLQYLEKLNLPESTHVEFVMFSTDVSHCVKD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SLFN5 Antibody - BSA Free
![Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]](https://resources.rndsystems.com/images/products/SLFN5-Antibody-Immunohistochemistry-Paraffin-NBP1-81178-img0016.jpg "Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]")
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes as expected.![Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]](https://resources.rndsystems.com/images/products/SLFN5-Antibody-Immunohistochemistry-Paraffin-NBP1-81178-img0013.jpg "Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]")
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]](https://resources.rndsystems.com/images/products/SLFN5-Antibody-Immunohistochemistry-Paraffin-NBP1-81178-img0014.jpg "Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]")
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.![Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]](https://resources.rndsystems.com/images/products/SLFN5-Antibody-Immunohistochemistry-Paraffin-NBP1-81178-img0015.jpg "Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]")
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human endometrium shows strong nuclear positivity in glandular cells.A![Simple Western: SLFN5 Antibody [NBP1-81178]](https://resources.rndsystems.com/images/products/SLFN5-Antibody-Simple-Western-NBP1-81178-img0006.jpg "Simple Western: SLFN5 Antibody [NBP1-81178]")
Simple Western: SLFN5 Antibody [NBP1-81178]
Simple Western: SLFN5 Antibody [NBP1-81178] - Simple Western lane view shows a specific band for SLFN5 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: SLFN5 Antibody [NBP1-81178]](https://resources.rndsystems.com/images/products/SLFN5-Antibody-Simple-Western-NBP1-81178-img0007.jpg "Simple Western: SLFN5 Antibody [NBP1-81178]")
Simple Western: SLFN5 Antibody [NBP1-81178]
Simple Western: SLFN5 Antibody [NBP1-81178] - Electropherogram image(s) of corresponding Simple Western lane view. SLFN5 antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).![SLFN5 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: SLFN5 Antibody [NBP1-81178]](https://resources.rndsystems.com/images/products/nbp1-81178_-immunocytochemistry-immunofluorescence-639174076358819374.jpg "Immunocytochemistry/ Immunofluorescence: SLFN5 Antibody [NBP1-81178]")
Immunocytochemistry/ Immunofluorescence: SLFN5 Antibody [NBP1-81178]
Staining of human cell line A-431 shows localization to nucleoplasm & vesicles.Applications for SLFN5 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Simple Western
1:20
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 92 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 92 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLFN5
Alternate Names
MGC150611, MGC150612, MGC19764, schlafen family member 5
Entrez Gene IDs
162394 (Human)
Gene Symbol
SLFN5
Additional SLFN5 Products
Product Documents for SLFN5 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SLFN5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SLFN5 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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