SMPD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-22365

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMPD1 Primary Antibodies »

Product Specifications

Immunogen

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 391-419 in the C-terminal region of human SMPD1 (UniProt Accession #P17405). Species sequence homology: bovine

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Scientific Data Images for SMPD1 Antibody - BSA Free

Western Blot: SMPD1 Antibody [NBP2-22365]

Western Blot: SMPD1 Antibody [NBP2-22365]

Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate |Lysates/proteins at 20 ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size: 70kD Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]

Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]

Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemical analysis of paraffin-embedded human breast tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody..
Flow Cytometry: SMPD1 Antibody [NBP2-22365]

Flow Cytometry: SMPD1 Antibody [NBP2-22365]

Flow Cytometry: SMPD1 Antibody [NBP2-22365] - Indirect Flow Cytometric analysis of K562 cells (right histogram) using SMPD1 antibody compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot: SMPD1 Antibody [NBP2-22365]

Western Blot: SMPD1 Antibody [NBP2-22365]

Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: human cerebellum lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG, (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 70kD |Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]

Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365]

Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemistry analysis of formalin fixed paraffin embedded human testis tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody

Applications for SMPD1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:25

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:4000
Application Notes
Immunohistochemistry (FFPE): Requires HIER using EDTA, pH 8.0 buffer pH9.0 prior to staining. Optimal dilutions to be determined by the researcher.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMPD1

The protein encoded by the SMPD1 gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Three transcript variants encoding two different isoforms have been found for this gene. (provided by RefSeq)

Long Name

Sphingomyelin Phosphodiesterase 1, Acid Lysosomal

Alternate Names

aSMase, NPD

Gene Symbol

SMPD1

UniProt

P17405 (Human)

Additional SMPD1 Products

Product Documents for SMPD1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SMPD1 Antibody - BSA Free

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Bioactive Lipids and Receptors

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Protocols

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FAQs

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