SMURF1 Antibody (1D7) - Azide and BSA Free

Novus Biologicals | Catalog # H00057154-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D7

Format

Azide and BSA Free
View all SMURF1 Primary Antibodies »

Product Specifications

Immunogen

SMURF1 (NP_065162, 165 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIP

Specificity

SMURF1 (1D7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SMURF1 Antibody (1D7) - Azide and BSA Free

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HepG2.
Immunocytochemistry/ Immunofluorescence: SMURF1 Antibody (1D7) [H00057154-M01]

Immunocytochemistry/ Immunofluorescence: SMURF1 Antibody (1D7) [H00057154-M01]

Immunocytochemistry/Immunofluorescence: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SMURF1 Antibody (1D7) [H00057154-M01]

Immunohistochemistry-Paraffin: SMURF1 Antibody (1D7) [H00057154-M01]

Immunohistochemistry-Paraffin: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 2 ug/ml.
Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 expression in Raw 264.7 ( Cat # L024V1 ).
Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - SMURF1 monoclonal antibody (M01), clone 1D7. Analysis of SMURF1 expression in HeLa.
Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01]

Western Blot: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody (M01), clone 1D7.Lane 1: SMURF1 transfected lysate(83.4 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: SMURF1 Antibody (1D7) [H00057154-M01]

Immunoprecipitation: SMURF1 Antibody (1D7) [H00057154-M01]

Immunoprecipitation: SMURF1 Antibody (1D7) [H00057154-M01] - Analysis of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SMURF1 MaxPab rabbit polyclonal antibody.
ELISA: SMURF1 Antibody (1D7) [H00057154-M01]

ELISA: SMURF1 Antibody (1D7) [H00057154-M01]

ELISA: SMURF1 Antibody (1D7) [H00057154-M01] - Detection limit for recombinant GST tagged SMURF1 is approximately 0.1ng/ml as a capture antibody.

Applications for SMURF1 Antibody (1D7) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMURF1

This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined.

Long Name

SMAD Ubiquitination Regulatory Factor 1

Alternate Names

EC 6.3.2, KIAA1625

Entrez Gene IDs

57154 (Human)

Gene Symbol

SMURF1

OMIM

605568 (Human)

UniProt

NP_065162 (Human)

Additional SMURF1 Products

Product Documents for SMURF1 Antibody (1D7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SMURF1 Antibody (1D7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SMURF1 Antibody (1D7) - Azide and BSA Free

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Protocols

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FAQs

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