SnoN Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77306

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SnoN/Ski-like Protein Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN. The immunogen is located within the first 50 amino acids of SnoN. Amino Acid Squence: KKLNGMGDDGSPPAKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SnoN Antibody - BSA Free

Western Blot: SnoN AntibodyBSA Free [NBP1-77306]

Western Blot: SnoN AntibodyBSA Free [NBP1-77306]

Western Blot: SnoN Antibody [NBP1-77306] - Analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry-Paraffin: SnoN Antibody - BSA Free [NBP1-77306]

Immunohistochemistry-Paraffin: SnoN Antibody - BSA Free [NBP1-77306]

Immunohistochemistry-Paraffin: SnoN Antibody [NBP1-77306] - Mouse lung tissue with SnoN antibody at 5 ug/ml.

Immunocytochemistry/Immunofluorescence: SnoN Antibody [NBP1-77306] -

Immunocytochemistry/Immunofluorescence: SnoN Antibody [NBP1-77306] - HeLa cells stained with SnoN antibody. Image from verified customer review.
SnoN Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SnoN Antibody - BSA Free [NBP1-77306] -

Immunocytochemistry/ Immunofluorescence: SnoN Antibody - BSA Free [NBP1-77306] - Immunofluorescence of SnoN in Mouse Lung cells with SnoN antibody at 20 ug/mL.

Applications for SnoN Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-1 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP1-77306 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SnoN/Ski-like Protein

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).

Long Name

SKI-like Oncogene

Alternate Names

Ski like Protein, SKIL, SNO, SnoA, SnoI

Gene Symbol

SKIL

UniProt

NP_005405 (Human)

Additional SnoN/Ski-like Protein Products

Product Documents for SnoN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SnoN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SnoN Antibody - BSA Free

Customer Reviews for SnoN Antibody - BSA Free (1)

4 out of 5
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Customer Images

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Hela cell line
    Species: Human
    Verified Customer | Posted 10/02/2015
    SnoN on HeLa
    SnoN Antibody - BSA Free NBP1-77306

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Protocols

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FAQs

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