SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

Novus Biologicals | Catalog # NBP1-22417

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4GG11G6

Format

BSA Free
View all SOD3/EC-SOD Primary Antibodies »

Product Specifications

Immunogen

Human extracellular SOD purified from aortas

Localization

Extracellular Space

Specificity

Detects extracellular SOD approx 35kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417]

Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417]

Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteo-arthritis..
Immunohistochemistry: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417]

Immunohistochemistry: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417]

Immunohistochemistry: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6. Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000.

Applications for SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of SOD (EC) Antibody was sufficient for detection of EC-SOD in 20 ug of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD3/EC-SOD

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Long Name

Superoxide Dismutase-3

Alternate Names

EC-SOD, ECSOD

Entrez Gene IDs

6649 (Human)

Gene Symbol

SOD3

UniProt

P08294

Additional SOD3/EC-SOD Products

Product Documents for SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

Certificate of Analysis

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Product Specific Notices for SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Citations for SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SOD3/EC-SOD Antibody (4GG11G6) - BSA Free

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  • Q: I've been using your mouse monoclonal anti-SOD3 antibody for a number of applications and have been very pleased with the results. However, I want to know what would be a good positive control for WB. The datasheet says that human aorta was used as the immunogen. However, I read that lung and kidney lysates make good positive controls for other anti-SOD3 antibodies. Would lung/kidney tissue work as a positive control for this particular antibody?

    A: SOD3 protein is mainly expressed in blood vessels, heart, lung, kidney and placenta, and it is the major SOD isoenzyme found in extracellular fluids such as plasma, lymph and synovial fluid. So using lung or kidney as a positive control lysate would be appropriate for your immunoassays including Western blot.

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