SOX10 Antibody (SD204-04)

Novus Biologicals | Catalog # NBP2-67812

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SD204-04 expressed in HEK293
View all SOX10 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide Human SOX10 aa 411-460 / 466. (SwissProt: P56693 Human; SwissProt: Q04888 Mouse; SwissProt: O55170 Rat)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX10 Antibody (SD204-04)

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunocytochemistry/Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunocytochemistry/Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Analysis of paraffin-embedded mouse brain tissue using anti-SOX10 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Analysis of paraffin-embedded rat brain tissue using anti-SOX10 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812]

Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for SOX10 Antibody (SD204-04)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

SRY-box transcription factor 10

Alternate Names

DOM, PCWH, SOX-10, WS2E, WS4, WS4C

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (SD204-04)

Certificate of Analysis

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Product Specific Notices for SOX10 Antibody (SD204-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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