SOX2 Antibody (SOX2/1791)

Novus Biologicals | Catalog # NBP2-53407

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SOX2/1791
View all SOX2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary) (Uniprot: P48431)

Localization

Nuclear

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54594)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for SOX2 Antibody (SOX2/1791)

Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot Analysis of human A549 cell lysate using SOX2 Antibody (SOX2/1791).
Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Intestine stained with SOX2 Antibody (SOX2/1791).
Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot of Recombinant SOX2 protein fragment and NCCIT cell lysate using SOX2 Antibody (SOX2/1791).
Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with SOX2 Monoclonal Antibody (SOX2/1791).
Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with SOX2 Antibody (SOX2/1791).
Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Stomach stained with SOX2 Antibody (SOX2/1791).
Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX2 Antibody (SOX2/1791).
Protein Array: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Protein Array: SOX2 Antibody (SOX2/1791) [NBP2-53407]

Protein Array: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX2 Antibody (SOX2/1791) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for SOX2 Antibody (SOX2/1791)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SOX2

The SOX-2 protein is a transcription factor that is critical for early embryogenesis and for embryonic stem cell pluripotency. The SOX-2 protein is also significant for eye development. SOX 2, also known as SRY related HMG BOX gene 2, belongs to the SOX (SRY-box containing gene) gene family. The SOX-2 protein is a transcription factor since it binds to DNA and regulates activity of other genes. All SOX family proteins share HMG box domains for DNA binding.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Gene Symbol

SOX2

UniProt

P48431

Additional SOX2 Products

Product Documents for SOX2 Antibody (SOX2/1791)

Certificate of Analysis

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Product Specific Notices for SOX2 Antibody (SOX2/1791)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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