SPG7 Antibody (HL2236) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25694

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2236 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fragmemt of human SPG7.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPG7 Antibody (HL2236) - Azide and BSA Free

SPG7 Antibody (HL2236) - Azide and BSA Free

Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] -

Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SPG7 Antibody (HL2236) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] -

Immunocytochemistry/Immunofluorescence: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI.
SPG7 Antibody (HL2236) - Azide and BSA Free

Immunohistochemistry-Paraffin: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] -

Immunohistochemistry-Paraffin: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:20. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SPG7 Antibody (HL2236) - Azide and BSA Free

Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] -

Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SPG7 Antibody (HL2236) - Azide and BSA Free

Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] -

Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for SPG7 Antibody (HL2236) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:20-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPG7

SPG7 - spastic paraplegia 7, paraplegin (pure and complicated autosomal recessive)

Alternate Names

CARMGC126331, cell matrix adhesion regulator, CMARMGC126332, EC 3.4.24, EC 3.4.24.-, FLJ37308, paraplegin, PGNcell adhesion regulator, spastic paraplegia 7 (pure and complicated autosomal recessive), Spastic paraplegia 7 protein, SPG5C

Gene Symbol

SPG7

Additional SPG7 Products

Product Documents for SPG7 Antibody (HL2236) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SPG7 Antibody (HL2236) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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