SPG7 Antibody (OTI1C1)

Novus Biologicals | Catalog # NBP2-01860

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C1
View all SPG7 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) produced in E.coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

88.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPG7 Antibody (OTI1C1)

Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860]

Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860]

Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860]

Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860]

Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860]

Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860]

Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860]

Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860]

SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0007.jpg
Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860]

Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860]

Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860]

Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860]

Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.
Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860]

Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860]

Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for SPG7 Antibody (OTI1C1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500 - 2000
Application Notes
Use in IHC reported in scientific literature (PMID 28131770).

Reviewed Applications

Read 1 review rated 4 using NBP2-01860 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPG7

This gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7. [provided by RefSeq].

Alternate Names

CARMGC126331, cell matrix adhesion regulator, CMARMGC126332, EC 3.4.24, EC 3.4.24.-, FLJ37308, paraplegin, PGNcell adhesion regulator, spastic paraplegia 7 (pure and complicated autosomal recessive), Spastic paraplegia 7 protein, SPG5C

Entrez Gene IDs

6687 (Human)

Gene Symbol

SPG7

Additional SPG7 Products

Product Documents for SPG7 Antibody (OTI1C1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SPG7 Antibody (OTI1C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SPG7 Antibody (OTI1C1)

Customer Reviews for SPG7 Antibody (OTI1C1) (1)

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  • SPG7 Antibody (OTI1C1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse liver lysate
    Species: Mouse
    Verified Customer | Posted 11/22/2020
    Mouse liver lysate processed and SPG7 stained. Primary antibody 1:1000 in Milk 5% in PBST (0.1% Tween) O/N 4ºC. Secondary ab Donkey anti-mouse Alexa fluor 555 1:5000 1h RT. Band was detected around 75 kDa.
    Mouse liver lysate processed and loaded in 12% TGX gel in reduced and denaturalization conditions. Milk 5% blocking in PBST (0.1% Tween) 1h. Primary antibody 1:1000 in Milk 5% in PBST (0.1% Tween) O/N 4ºC. Secondary Donkey anti-mouse Alexa fluor 555 1:5000 1h RT. Band observed around 75 kDa.
    SPG7 Antibody (OTI1C1) NBP2-01860

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