Spi-B Antibody (4G5)

Novus Biologicals | Catalog # NBP2-37458

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G5
View all Spi-B Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Spi-B (AA: 200-252) expressed in E. Coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Spi-B Antibody (4G5)

Western Blot: Spi-B Antibody (4G5)BSA Free [NBP2-37458]

Western Blot: Spi-B Antibody (4G5)BSA Free [NBP2-37458]

Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

Immunocytochemistry/ Immunofluorescence: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

Immunocytochemistry/Immunofluorescence: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded esophageal cancerwith DAB staining.
Flow Cytometry: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

Flow Cytometry: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

Flow Cytometry: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).
Western Blot: Spi-B Antibody (4G5)BSA Free [NBP2-37458]

Western Blot: Spi-B Antibody (4G5)BSA Free [NBP2-37458]

Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)
Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.
ELISA: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

ELISA: Spi-B Antibody (4G5) - BSA Free [NBP2-37458]

ELISA: Spi-B Antibody (4G5) [NBP2-37458] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Spi-B Antibody (4G5)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spi-B

SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).[supplied by OMIM]

Long Name

Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B

Alternate Names

SpiB

Gene Symbol

SPIB

Additional Spi-B Products

Product Documents for Spi-B Antibody (4G5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Spi-B Antibody (4G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Spi-B Antibody (4G5)

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail